Sarcandra in vitro adventitious bud-inducing factor screening bundle of.docVIP

 PAGE \* MERGEFORMAT 7 Sarcandra in vitro adventitious bud-inducing factor screening bundle of Author: Pan See Pan Chao-Mei Chen Huan Jian Huang Chong Cai Lai Chen-chen 【Abstract】 【Objective】 To investigate the impact of grass cultivation of coral adventitious bud induction factors for the early establishment of grass on behalf of the coral provide the basis for in vitro culture system. 【Methods】 caoshanhu hypocotyls to induce regeneration of seedlings obtained by sterile stem segments as explants to study 6  benzylaminopurine (6  BA) and indole butyric acid (IBA) with different concentration ratio, illumination time and intensity and temperature on the grass coral in vitro high-frequency induction of adventitious buds. 【Results】 6  BA and IBA combinations can effectively improve the grass coral adventitious buds induction rate, while high concentrations of plant hormones can inhibit the growth of adventitious buds Sarcandra; grass coral scattered light or weak growth and momentum under the direct light exposure is good, dark cultures or too strong direct light plants will appear yellow, shrink, or even death phenomena; at a temperature of 25℃, the grass coral adventitious bud induction rate of 100 %, less than 25℃ or above 30℃ are not conducive to their growth. 【Conclusion】 The MS medium as the basic medium supplemented with 2  5mg / L 6  BA 0  1mg / L IBA, a temperature of 25℃, with soft natural light scattering or below  2000lx  direct light exposure, time of less than 8h is effective in promoting grass coral in vitro induction of adventitious buds induction rate was 82  98 %.  Keywords: grass coral / growth and development; adventitious buds Cong; medium conditioned  Sarcandra  Sarcandra glabra  (Thumb ) Nakai, is a genus of coral Chloranthaceae Chloranthaceae grass  Sarcandra , also known as 9 tea Sarcandrae, elder, for the perennial herb or sub-shrubs. Sarcandra with anti-inflammatory, heat, detoxification and Shujinh