Sarmentosum Determination of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 11 Sarmentosum Determination of total flavonoids [Abstract] Objective To establish sarmentosum in the determination of total flavonoids. Methods ultraviolet spectrophotometry to measure wavelength of 501 nm. The results of testing the concentration of total flavonoids 0.010 36 ~ 0.082 88 mg / mL and the absorbance within the framework of a good linear relationship (r = 0.9998), the average recovery was 98.62% (RSD = 1.11%, n = 6). Conclusion This method is used to sarmentosum Determination of total flavonoids, accurate, reliable and stable. [Keywords:] UV spectrophotometry; sarmentosum; total flavonoids; Determination Sarmentosum (Sedum sarmentosum Bunge) also known as horizontal stems Sedum, herbaceous perennial Sedum Department sarmentosum full of fresh or dry grass, are distributed in most parts of China. Drug sweet, light, sexual cool, with heat-clearing and detoxifying, diuretic swelling, removing the abscess myogenic effect, attending erysipelas ulcers, negative urine and acute and chronic hepatitis. According to the literature reports, sarmentosum contain cyanogenic glycosides, flavonoids, steroids and triterpenoids and other compounds [1,2]. Pan fire, etc. [3,4] on the Liver sarmentosum JiangMei active ingredient conducted a series of studies to prove that sarmentosum JiangMei has a liver protection effect of the active ingredient found primarily in H2O part and n  BuOH portion ( main-containing glycosides and total flavonoids), and then verify that the total flavonoids are sarmentosum JiangMei in liver protection, one of the main active component. In this paper, the classical colorimetric right sarmentosum of total flavonoids were determined, believing that its successors provide the basis for the development and utilization. An instrument and reagent UV  2401 type ultraviolet visible spectrophotometer (Shimadzu, Japan); bp211D type one hundred thousandth balance (Sartorius Corporation); Soxhlet extractor and so on. R