Selective COX inhibitor NS3982 down K562-ADM cells MDR1 - Pgp expression.docVIP

 PAGE \* MERGEFORMAT 12 Selective COX inhibitor NS3982 down K562/ADM cells MDR1 / Pgp expression OF: How the fire Cong, Thuringia, Ying Su, Jia Jing, Zou Im, Lin Hua-mei [Abstract] Objective To investigate the selective cyclooxygenase-2 (COX 2 inhibitor NS 398 K562/ADM on leukemia cell multidrug resistance P glycoprotein (P gp expression. Methods Cell K562/ADM role for the NS 398 target cells, MTT cell proliferation assay, reverse transcription polymerase chain reaction (RT PCR detection of multidrug resistance gene (MDRlmRNA expression by flow cytometry (FCM determination of P gp protein expression. Results NS 398 significantly suppressed K562 / ADM cell proliferation, time and dose-related effect is, down K562/ADM cell MDRl gene expression and inhibits P gp synthesis, showed a dose dependent relationship. Conclusion COX 2 inhibitor NS 398 in a certain time and dose range inhibit the Leukemia drug resistance K562/ADM cells MDRl / P gp expression, and inhibited the MDR cell K562/ADM value. [Keywords:] NS 398 K562/ADM cell multidrug resistance P glycoprotein COX (cyclooxygenase, COX) is catalyzed in vivo a key enzyme in prostaglandin synthesis. Subtype of COX 2 has been found and has close ties with many human tumors, may be involved in tumor proliferation, apoptosis, tumor angiogenesis and tumor invasion of, COX 2 treatment and prevention of cancer has become an important target for [12]. In recent years, studies have shown that, COX 2 may increase the multidrug resistance gene / P glycoprotein (MDR1 / P gp expression and MDR1 / P gp overexpression of tumor to radiotherapy and chemotherapy are not sensitive and led to an important cause of treatment failure [3]. Therefore, further clarify the COX 2 and MDR1 / P gp K562/ADM multidrug resistance in leukemia cells of relations, on the use of COX 2 inhibitors to reverse or prevent tumor drug resistance, increased sensitivity to radiotherapy and chemotherapy has important theoretical and clinical signifi