Three kinds of colon cancer cell lines DLC 1 gene expression and its promoter region methylation status of research.docVIP

 PAGE \* MERGEFORMAT 18 Three kinds of colon cancer cell lines DLC 1 gene expression and its promoter region methylation status of research 【Abstract】 Objective: To study the candidate tumor suppressor genes DLC  1 in colon cancer cell lines expression and promoter methylation status of the preliminary study of colon cancer cell lines DLC  1 gene expression and its promoter methylation of relevance. Methods: RT  PCR technique to detect DLC  1 gene in human colon cancer cell line CaCo  2, HT  29 and LoVo in the expression level; application of methylation-specific PCR (MSP) method and bisulfite modification DNA sequencing detected the methylation status of the promoter region; respectively 0,5,10 μmol * L-1 concentration of demethylation drug 5-azacytidine treatment DLC  1 gene promoter region hypermethylation status of cells After re-testing DLC  1 gene expression levels of mRNA. Results: The human colon cancer cell line CaCo  2 and LoVo in DLC  1 mRNA positive expression, HT  29 expression was negative expression; CaCo  2 and LoVo cells, DLC  1 gene promoter region methylation does not occur, The HT  29 appears in the promoter region hypermethylation state; by 5-azacytidine drug intervention, HT  29 colon cancer cells in DLC  1 gene expression was significantly increased. Conclusion: The colon cancer cell line HT  29 in the DLC  1 gene silencing and promoter hypermethylation status of the. Keywords: DLC  1 gene methylation in colon cancer cell lines by methylation-specific PCR DLC  1 (frequently deleted in liver, DLC  1) gene, located on human chromosome 8p21  22, may be a new tumor suppressor gene. 1998, Yuan et al [1] first used representational difference analysis technique (representational difference analysis, RDA) will be of primary liver cancer tissues from the separated and that the genetic change is one of the events early onset of liver cancer. Followed, DLC  1 gene function and in breast, lung, stomach and