DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria.docVIP

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DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria.doc

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DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria

BMC Genomics BioMedCentral Research article Open Access DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria James Pannucci, Richard T Okinaka, Erin Williams, Robert Sabin, Lawrence O Ticknor and Cheryl R Kuske* Address: Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA Email: James Pannucci - pannucci_james@; Richard T Okinaka - okinaka@; Erin Williams - erin@; Robert Sabin - sabin@; Lawrence O Ticknor - lot@; Cheryl R Kuske* - kuske@ * Corresponding author Published: 9 December 2002 Received: 19 June 2002 Accepted: 9 December 2002 BMC Genomics 2002, 3:34 This article is available from: /1471-2164/3/34 ? 2002 Pannucci et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity to B. anthracis, as determined by amplified fragment length polymorphism (AFLP) analysis, were examined by PCR for the presence of sequences similar to 47 pXO2 ORFs. Results: The two most distantly related isolates examined, B. thuringiensis 33679 and B. thuringiensis AWO6, produced the greatest number of ORF sequences similar to pXO2; 10 detected in 33679 and 16 in AWO6. No more than two of the pXO2 ORFs were detected in any one of the remaining isolates. Dot-blot DNA hybridizations between pXO2 ORF fragments and total genomic DNA from AWO6 were consistent with the PCR assay results for this isolate and also revealed nine additional ORFs shared between these two bacteria. Sequences similar to the B. anthracis cap genes or their regu