BML-111下调OPN抑制Hela细胞（宫颈癌）增殖和迁移.pdfVIP

Abstract ABSTRACT Objective: Human papilloma virus (HPV) in cervical chronic inflammation caused by persistent infection is the most important reason of cervical cancer. Lipoxins (lipoxins, LXs) is brake signal of inflammatory response [1,2]. It is speculated that lipoxin analogs (BML-111) may inhibit chronic inflammation ,which also suppress the occurrence of cervical cancer. The experiment proposed by lipopolysaccharide (LPS) in vitro inflammatory microenvironment, which is to explore the effect of BML-111 on the proliferation and migration in LPS-induced cervical cancer (Hela cell). And compared with the OPN gene silencing Hela cells group, the effect of proliferation and migration and invasion caused by BML-111 ,which was observed on LPS-induced Hela cell. The aim is to provide experimental evidence for the clinical treatment of cervical cancer. Methods: (1) Using MTT to detect the effect of viability caused by BML- 111 on LPS-induced Hela cell; (2) Using Western blot to detect the effect of BML-111 on the expression of OPN, P53, MMP2 and MMP 9 protein in LPS-induced Hela cells; (3) By transfecting ShRNA-OPN in human Hela cells ，the expression of OPN was inhibited. Using Western blot method again to detect the effect of BML- 111 on the expression of OPN, P53, MMP2 and MMP 9 protein in LPS-induced Hela cells; (4) Through scratches and Transwell experiments, we observed the the effect of BML-111 on the migration in LPS-induced Hela cells; Results ： (1) MTT analysis showed: at 24h and 48h in vitro, We are supposed to use different concentrations of LPS to stimulate Hela cells.As the control group, 100,10,1,0.1μg/mL LPS stimulation group OD values wer