γ分泌酶抑制剂阻断Notch信号通路对肺腺癌细胞A549增殖的影响.pdfVIP

Results: It is confirmed by Western-blot method and RT-PCR method that Notch1-4 subtyes were expressed in A549 cells ; under normoxic conditions , different concentration (5μM、10μM、20μM ) MW167 processing lung adenocarcinoma cell line A549 , after 48 hours , Notch1 protein expression in each dosing group compared with control group was elevated (P0.05) , with the MW167 concentration increasing , the Notch1 protein accumulation rate of increase did not increase correspondingly （r =0.2 ， P0.05 ） ; different concentration (5μM、10μM、20μM ) MW167 processing lung adenocarcinoma cell line A549 , after 48 hours , HES1 mRNA expression in each dosing group compared with control group was decreasd (P0.05) , the inhibition rate of HES1 mRNA express increased gradually , with the MW167 concentration increasing ( r = 0.927 ，P0.01) ; different concentration (5μM 、10μM、20μM )MW167 processing lung adenocarcinoma cell line A549 , after 48 hours , the absorbance A value by MTT method in eath dosing group was higher than the control group (P0.05) , proliferation rate increase with increasing concentration (r = 0.904,P0.0 1). Conclusions: 1. The Notch receptor subtypes were expressed in lung adenocarcinoma A549 cells , it provided the premise for the further research about relationship between Notch signaling pathway and lung adenocarcinoma A549 cells . 2. Under normoxic conditions , different concentration (5μM、10μM、20μM ) MW167 processing lung adenocarcinoma cell line A549 , after 48 hours , expression of Notch1 protein in dosing group was increased , but expression of downstream gene HES1 mRNA was decreased , it is explained that MW167 can block Notch1 protease cleavage activation in different degrees , and make Notch1 protein accumulated , increase expression of Notch1 , and inhibit the expre