WTX基因在肝癌组织和细胞株中的表达及临床意义.pdfVIP

Abstract ABSTRACT Objective: To investigate the expression level of WTX (Wilms Tumor gene on the X chromosome) gene in normal liver tissue,para-cancerous tissues and liver cancer tissues;To detect the expression of WTX gene in normal liver cells and hepatoma cells with different degree of malignancy; To analyze the relationship of WTX gene expression between normal liver cells and hepatoma cells in different degree of malignancy;To investigate the expression and significance of WTX gene in liver cancer. Methods: 1、We collected 40 patient’s liver cancer tissues and para-cancerous tissue (from the edge of the lesion 2cm of the normal liver tissues) from the patient who don ’t have other malignant tumor history, radiotherapy, chemotherapy and biological therapy before accepting operation treatment, setting up the hepatolithiasis and traumatic removal of part of normal liver tissue as a normal control group. 2 、The expression of WTX mRNA in the liver cancer tissues ,normal tissues and para-cancerous tissues was detected by semiquantitative RT-PCR. 3、The differential expression of WTX mRNA and protein in a normal liver cell line and different hepatoma cells line with different degree malignance were detected by RT-PCR and Western Blot ,and was analysed the relationship of WTX between different hepatoma cells line. Results: 1、The expression of WTX mRNA in hepatocellular carcinoma tissues with RT-PCR, the result suggested that cancer tissue was significantly lower than that of para-cancerous tissues and normal tissues, two the difference was statistically significant