Antifouling Activity of Bacterial Symbionts of Seagrasses against Marine Biofilm-Forming Bacteria英文文献资料.docVIP

Journal of Environmental Protection, 2011, 2, 1245-1249 doi:10.4236/jep.2011.29143 Published Online October 2011 (/journal/jep) Antifouling Activity of Bacterial Symbionts of Seagrasses against Marine Biofilm-Forming Bacteria Bintang Marhaeni , Ocky Karna Radjasa , Miftahuddin Majid Khoeri , Agus Sabdono , 1,2 3,4* 3 4 1 3 Dietriech G. Bengen , Herawati Sudoyo 1 Graduate School of Marine Sciences, Bogor Agricultural University, Bogor, West Java, Indonesia; Department of Fisheries and 2 Marine Science, Soedirman University, Purwokerto, Central Java, Indonesia; Marine Microbiology Unit, Eijkman Institute for Mo- Department of Marine Science, Diponegoro University, Semarang, Central Java, Indonesia. 3 lecular Biology, Jakarta, Indonesia; Email: ocky_radjasa@undip.ac.id 4 Received September 6 , 2011; revised October 5 , 2011; accepted November 3 , 2011. th th rd ABSTRACT Marine biofouling has been regarded as a serious problem in the marine environment. The application of TBT and other heavy metal-based antifoulants has created another environmental problem. The present study explored the possi- ble role of baterial symbionts of seagrasses Thalassia hemprichii, and Enhalus acoroides, which were successfully screened for antifouling activity against marine biofilm-forming bacteria isolated from the surrounding colonies of seagrasses. Bacterial symbionts were isolated and tested against biofilm-forming bacteria resulted in 4 bacterial sym- bionts capable of inhibiting the growth biofilm-forming isolates. Molecular identification based on 16S rRNA gene se- quences reveale