Antigenic and Genotypic Similarity between Primary Glioblastomas and Their Derived Neurospheres英文文献资料.docVIP

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Antigenic and Genotypic Similarity between Primary Glioblastomas and Their Derived Neurospheres英文文献资料.doc

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Antigenic and Genotypic Similarity between Primary Glioblastomas and Their Derived Neurospheres英文文献资料

HindawiPublishingCorporation JournalofOncology Volume2011,ArticleID314962,16pages doi:10.1155/2011/314962 ResearchArticle AntigenicandGenotypicSimilaritybetweenPrimary GlioblastomasandTheirDerivedNeurospheres ValentinaCaldera, 1 MartaMellai, 1 LauraAnnovazzi, 1AngelaPiazzi,1,2MicheleLanotte,3 4 PaolaCassoni, andDavideSchiffer 1 1 2 3 4 Neuro-Bio-OncologyCenter,PoliclinicodiMonzaFoundation,UniversityofTurin,13100Vercelli,Italy DepartmentofMedicalSciences,UniversityofPiemonteOrientale,28100Novara,Italy DepartmentofNeuroscience,NeurosurgicalUnit,UniversityofTurin,10124Turin,Italy DepartmentofBiomedicalSciencesandHumanOncology,UniversityofTurin,10124Turin,Italy CorrespondenceshouldbeaddressedtoDavideSchi?er,davide.schi?er@unito.it Received14February2011;Revised4April2011;Accepted6June2011 AcademicEditor:DominicFan Copyright?2011ValentinaCalderaetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Formationofneurospheres(NS)inculturesofglioblastomas(GBMs),withself-renewal,clonogeniccapacities,andtumorigenicity followingtransplantationintoimmunode?cientmice,maydenouncetheexistenceofbraintumorstemcells(BTSCs)invivo. In sixteen cell lines from resected primary glioblastomas, NS showed the same genetic alterations as primary tumors and the expression of stemness antigens. Adherent cells (AC), after adding 10% of fetal bovine serum (FBS) to the culture, were geneticallydi?erentfromNSandprevailinglyexpresseddi?erentiationantigens.NSdevelopedfromahighlymalignanttumor phenotypewithproliferation,circumscribednecrosis,andhighvesseldensity.Besideoriginatingfromtransformedneuralstem cells(NSCs),BTSCsmaybecontainedwithinorcorrespondtodedi?erentiatedcellsaftermutationaccumulation,whichreacquire theexpressionofstemnessantigens. 1.Introduction from tumor samples put in culture, of neurospheres (NS) and,byadding10%offetalbovineserum(FB