Applicability of Three Alternative Instruments for Food Authenticity AnalysisGMO Identification英文文献资料.docVIP

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Applicability of Three Alternative Instruments for Food Authenticity AnalysisGMO Identification英文文献资料.doc

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Applicability of Three Alternative Instruments for Food Authenticity AnalysisGMO Identification英文文献资料

SAGE-HindawiAccesstoResearch BiotechnologyResearchInternational Volume2011,ArticleID838232,8pages doi:10.4061/2011/838232 ResearchArticle ApplicabilityofThreeAlternativeInstrumentsfor FoodAuthenticityAnalysis:GMOIdenti?cation A.Burrell,C.Foy,andM.Burns MolecularandCellBiology,LGC,QueensRoad,Teddington,MiddlesexTW110LY,UK CorrespondenceshouldbeaddressedtoM.Burns,malcolm.burns@lgc.co.uk Received6July2010;Revised22November2010;Accepted19December2010 AcademicEditor:YvesBertheau Copyright?2011A.Burrelletal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Ensuring foods are correctly labelled for ingredients derived from genetically modi?ed organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examinesthe?tnessforpurposeoftheapplicationofthreelaboratoryelectrophoresisinstruments(AgilentBioanalyzer2100, Lab901TapeStation,andShimadzuMCE-202MultiNA)forthedetectionofGMOsusingPCRbasedonapreviouslyvalidated protocol.Whilstminordi?erencesintheperformancecharacteristicsofbiasandprecisionwereobserved,allthreeinstruments demonstratedtheirapplicabilityinusingthisprotocolforscreeningofGMOingredients. 1.Introduction of the local government for enforcement and legislative purposes.ManyDNAdetectionandscreeningprotocolsthat utiliseend-pointPCRandhavebeenappliedontheAgilent Bioanalyzer2100havebeenvalidatedandendorsedbythe UKFoodStandardsAgency(FSA).TheAgilentBioanalyzer 2100hasbeenshowntobeusefulasascreeningapproach inanumberoffoodauthenticitystudies[3]andcano?er analternativeapproachcomparedtoinstrumentsusingthe “goldstandard”ofreal-timePCR,thatcanbeprohibitively expensivetopurchaseintermsofinitial?nancialoutlayand costsassociated