Applicability of the P19CL6 cells as a model of cardiomyocytes – a transcriptome analysis英文文献资料.docVIP

Vol.2, No.1, 24-31 (2010) Health doi:10.4236/health.2010.21005 Applicability of the P19CL6 cells as a model of cardiomyocytes – a transcriptome analysis 1, 2 2 3 2* Iraj Khodadadi , Nick J. Plant , Vassilis Mersinias , Alfred E. Thumser 1 2 3 Department of Biochemistry and Nutrition, Hamedan University of Medical Sciences, Hamedan, Iran Division of Biochemical Sciences, University of Surrey, Guildford, United Kingdom B.S.R.C. “Alexander Fleming”, Varkiza, Greece; a.thumser@surrey.ac.uk Received 15 November 2009; revised 4 December 2009; accepted 7 December 2009. ABSTRACT issues has been the utilisation of chemically-stimulated differentiation of stem cells, with the hope that these cell lines will have a more realistic phenotype than cell lines derived from fully differentiated tissue. Pluripotent em- bryonic carcinoma cells have been reported as successful in vitro models of cardiac differentiation; for example, the P19 mouse embryonal carcinoma cell-line has been re- ported to differentiate into an embryonic cardiac-muscle phenotype in vitro [1] upon the addition of dimethyl sulfoxide (DMSO) [2-5]. Differentiated P19 cells have been reported to retain the ability to spontaneously con- tract and shown to express transcripts in a temporal manner during culture, suggestive of a cardiac-muscle phenotype [5-7], and as such these cells have therefore been extensively used to study cardiac cell physiology [1,2,5,6,8], although with the caveat that these cells are embryonic instead. However, in addition to these cardiac- muscle-specific properties, P19 cells also display pluri- potent properties and can be differentiated into cells dis- playing either a skeletal muscle or neural phenotype [1, 3-5]. There has thus been some concern about the ho- mogeneity of