Application of quartz crystal nanobalance in conjunction with a net analyte signal based method for simultaneous determination of leucine, isoleucine and valine英文文献资料.docVIP

J. Biomedical Science and Engineering, 2009, 2, 532-537 JBiSE doi: 10.4236/jbise.2009.27077 Published Online November 2009 (http://www.SciRP.org/journal/jbise/). Application of quartz crystal nanobalance in conjunction with a net analyte signal based method for simultaneous determination of leucine, isoleucine and valine Maryam Shojaei , Abdolreza Mirmohseni , Maryam Farbodi 1 2 2 1 Department of Natural sciences, Faculty of Animal sciences, University of Tabriz, Tabriz, Iran; Polymer Research Technology Labo- 2 ratory, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. Email: mshojaei@tabrizu.ac.ir; mirmohseni@tabrizu.ac.ir; farbodi@tabrizu.ac.ir Received 22 June 2009; revised 16 July 2009; accepted 19 July 2009. ABSTRACT retardation if not detected soon after birth and l-leucine and/or its keto acid are considered to be the main neu- rotoxic metabolites in MSUD. So, identification and detection of the abnormal levels of these metabolites in urine samples are necessary to diagnosis and therapy of these pathologies [1]. MSUD screening methods based on gas chromatog- raphy-mass spectrometry (GC-MS) and tandem mass spectrometry (MS-MS) have been developed. MS-MS has been reported to be a powerful diagnostic tool in MSUD patients. GC-MS and MS-MS have high resolu- tions that enable them to be used to measure several amino acids simultaneously. However, these instruments are prohibitively expensive, and hospitals in developing countries cannot afford it [2]. In general, the high-performance liquid chromatogra- phy (HPLC) method has been devised to measure amino acid levels, but developed methods have disadvantages, which include complex sample preparation and long analysis time [3]. Mass screening emergency for MSUD in childhood demands to