Biological Evaluation of Tissue-Engineered Cartilage Using Thermoresponsive Poly(N-isopropylacrylamide)-Grafted Hyaluronan英文文献资料.docVIP

Journal of Biomaterials and Nanobiotechnology, 2012, 3, 1-9 1 /10.4236/jbnb.2012.31001 Published Online January 2012 (http://www.SciRP.org/journal/jbnb) Biological Evaluation of Tissue-Engineered Cartilage Using Thermoresponsive Poly(N-isopropylacrylamide)-Grafted Hyaluronan Kazuaki Muramatsu, Mika Ide, Fujio Miyawaki Division of Life Science and Engineering, School of Science and Engineering, Tokyo Denki University, Saitama, Japan. Email: k-muramatsu@mail.dendai.ac.jp Received September 28 , 2011; revised November 29 , 2011; accepted December 14 , 2011 th th th ABSTRACT In order to contribute to the development of minimally invasive surgery techniques for autologous chondrocyte implan- tation, a novel self-assembling biomaterial consisting of thermoresponsive poly(N-isopropylacrylamide)-grafted hyalu- ronan (PNIPAAm-g-HA) has been synthesized as an injectable scaffold for cartilage tissue engineering. The aim of this study was to investigate the efficacy and cytocompatibility of PNIPAAm-g-HA to normal chondrocytes by using re- verse transcription-polymerase chain reaction (RT-PCR) analysis and histochemical staining in preliminary in vitro and in vivo experiments. Hematoxylin and eosin staining showed homogeneous distribution of cells in the PNIPAAm-g-HA hydrogel in 3-dimensional in vitro cultivation. Alcian blue staining also indicated that abundant extracellular matrix formation, including acidic glycosaminoglycans, occurred in tissue-engineered cartilage over time in vitro. Cartilage- related gene expression patterns, which were tested in rabbit normal chondrocytes embedded in the hydrogel, were al- most maintained for 4 weeks. Transforming growth factor-β1 (TGF-β1) stimulation enhanced the expression of SRY-related HMG box-containing gene 9 (Sox9) and type X collagen gen