Biosurfactant-Producing Lactobacilli Screening, Production Profiles, and Effect of Medium Composition英文文献资料.docVIP

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Biosurfactant-Producing Lactobacilli Screening, Production Profiles, and Effect of Medium Composition英文文献资料.doc

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Biosurfactant-Producing Lactobacilli Screening, Production Profiles, and Effect of Medium Composition英文文献资料

HindawiPublishingCorporation AppliedandEnvironmentalSoilScience Volume2011,ArticleID201254,9pages doi:10.1155/2011/201254 ResearchArticle Biosurfactant-ProducingLactobacilli:Screening,Production Pro?les,andEffectofMediumComposition EduardoJ.Gudi?na,Jos′eA.Teixeira,andL′?giaR.Rodrigues InstituteforBiotechnologyandBioengineering(IBB),CentreofBiologicalEngineering,UniversityofMinho, CampusdeGualtar,4710-057Braga,Portugal CorrespondenceshouldbeaddressedtoEduardoJ.Gudi?na,egudina@deb.uminho.pt Received22June2011;Accepted28September2011 AcademicEditor:ElenaTamburini Copyright?2011EduardoJ.Gudi?naetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell- bound biosurfactants) was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4mNm?1 and 22.0mNm?1, respectively. Biosurfactant production by this strainwasevaluatedunderdi?erentculturebrothcompositions.Theuseofdi?erentnitrogensourcesrevealedthatyeastextract isessentialforbacterialgrowth,whilepeptoneiscrucialforbiosurfactantsynthesis.Forbiosurfactantproduction,theuseof peptoneandmeatextractyieldedahigherproductionwhencomparedtothestandardmedium,withasurfacetensionreduction of24.5mNm?1.Furthermore,experimentswerealsoconductedina reactorwith pHandtemperaturecontrol.Biomassand biosurfactantproductioninbioreactorwashighercomparingwiththeexperimentsconductedinshake?aks.Theoptimization procedureadoptedinthecurrentworkwasfoundtoimprovethebiosurfactantproductionandopenednewperspectivesforthe useofL.paracaseissp.paracaseiA20asapromisingbiosurfactant-producer. 1.Introduction survival strategy and appears to have evolved in an inde- pendent, yet parallel fashion [6, 7]. A num