Carbonic anhydrase VII–a potential prognostic marker in gliomas英文文献资料.docVIP

Vol.3, No.1, 6-12 (2011) Health doi:10.4236/health.2011.31002 Carbonic anhydrase VII–a potential prognostic marker in gliomas Fatemeh Bootorabi , Joonas Haapasalo , Elona Smith , Hannu Haapasalo , 1,2 3 1 3 Seppo Parkkila 1,2 1 2 3 Institute of Medical Technology and School of Medicine, University of Tampere, Tampere, Finland Department of Clinical Chemistry, Centre for Laboratory Medicine, Tampere University Hospital, Tampere, Finland Department of Pathology, Centre for laboratory Medicine, Tampere University Hospital, Tampere, Finland; *Corresponding Author: seppo.parkkila@uta.fi Received 7 December 2010; revised 20 December 2010; accepted 26 December 2010 ABSTRACT Oligodendroglioma; pH Carbonic anhydrase VII (CA VII) is a cytosolic enzyme expressed in several organs, including the human brain, but it has not been investi- gated earlier in any tumors. We designed the present study to evaluate CA VII expression in a cohort of human diffuse astrocytomas, mixed oligoastrocytomas and oligodendrogliomas. CA VII immunostaining was correlated to clinico- pathologic findings, survival data, and expres- sion of other molecular factors, including Ki-67, p53 protein and epidermal growth factor recep- tor. CA VII-positive staining was observed in 94% of astrocytomas and 85% of oligodendrog- liomas. In the tumor specimens, strong positive areas were often located in close proximity to necrosis. The CA VII immunoreactivity showed positive correlation with tumor malignancy grades of astrocytomas (p = 0.02, chi-square test). In all tumor categories, CA VII-positive staining was often seen in the endothelial cells of neovessels in addition to the tumor cells. CA VII intensity showed no significant association with p53 nor did it correlate with the a