Efficient Isolation of Mouse Liver NKT Cells by Perfusion 英文参考文献.docVIP

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Efficient Isolation of Mouse Liver NKT Cells by Perfusion 英文参考文献.doc

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Efficient Isolation of Mouse Liver NKT Cells by Perfusion 英文参考文献

EfficientIsolationofMouseLiverNKTCellsbyPerfusion XianfengFang1,2,PeishuangDu1,YangLiu3*,JieTang1* 1CenterforInfectionandImmunity,InstituteofBiophysics,ChineseAcademyofScience,Beijing,China,2GraduateUniversityofChineseAcademyofSciences,Beijing, China,3DepartmentsofSurgery,InternalMedicineandPathology,UniversityofMichiganatAnnArbor,AnnArbor,Michigan,UnitedStatesofAmerica Abstract Background: NKT cellis apopulationof unconventionalTcells thatmediateboth innateandadaptive Tcellresponses. SinceNKTcellsaremostabundantintheliver,muchofNKTbiologyhasbeenlearntfromstudiesofNKTcellsisolatedfrom liver.Thisisacumbersomeprocedurewithvariationsincellyield. Results:BasedonrecentevidencethatNKTcellsresideinlivervascularcompartment,wedevelopedasimplemethodto isolateNKTcellsbyperfusionwithPBS-containing10mMofEDTA.ThenumberandcellsurfacephenotypeofliverNKTcells recovered by perfusion and by the traditional method were comparable. The yield of other lymphocytes was also comparable. Conclusion/Significance: Our data demonstrated that liver lymphocytes can be efficiently isolated by simple perfusion. These data provide a convenient method to isolate liver lymphocyte while preserving liver tissue for other analysis. Citation:FangX,DuP,LiuY,TangJ(2010)EfficientIsolationofMouseLiverNKTCellsbyPerfusion.PLoSONE5(4):e10288.doi:10.1371/journal.pone.0010288 Editor:DeryaUnutmaz,NewYorkUniversity,UnitedStatesofAmerica ReceivedMarch9,2010;AcceptedMarch29,2010;PublishedApril21,2010 Copyright: ? 2010 Fang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisstudyissupportedbytheChineseNaturalScienceFoundationandUnitedStatesNationalInstitutesofHealth.Thefundershadnoroleinstudy design,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetin