Electrophysiological Properties of Embryonic Stem Cell-Derived Neurons 英文参考文献.docVIP

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Electrophysiological Properties of Embryonic Stem Cell-Derived Neurons 英文参考文献.doc

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Electrophysiological Properties of Embryonic Stem Cell-Derived Neurons 英文参考文献

ElectrophysiologicalPropertiesofEmbryonicStemCell- DerivedNeurons JessicaR.Risner-Janiczek*,MarkA.Ungless,MengLi MedicalResearchCouncil,ClinicalSciencesCentre,FacultyofMedicine,ImperialCollege,London,UnitedKingdom Abstract Invitrogenerationoffunctionalneuronsfromembryonicstem(ES)cellsandinducedpluripotentstemcellsoffersexciting opportunitiesfordissectinggenefunction,diseasemodelling,andtherapeuticdrugscreening.Torealizethepotentialof stem cells in these biomedical applications, a complete understanding of the cell models of interest is required. While rapid advances have been made in developing the technologies for directed induction of defined neuronal subtypes, most published works focus on the molecular characterization of the derived neural cultures. To characterize the functionalpropertiesoftheseneuralcultures,weutilizedanEScellmodelthatgaverisetoneuronsexpressingthegreen fluorescentprotein(GFP)andconductedtargetedwhole-cellelectrophysiologicalrecordingsfromEScell-derivedneurons. Current-clamp recordings revealed that most neurons could fire single overshooting action potentials; in some cases multiple action potentials could be evoked by depolarization, or occurred spontaneously. Voltage-clamp recordings revealed that neurons exhibited neuronal-like currents, including an outward current typical of a delayed rectifier potassium conductance and a fast-activating, fast-inactivating inward current, typical of a sodium conductance. Taken + together,theseresultsindicatethatEScell-derivedGFP neuronsinculturedisplayfunctionalneuronalpropertiesevenat early stages of differentiation. Citation: Risner-Janiczek JR, Ungless MA, Li M (2011) Electrophysiological Properties of Embryonic Stem Cell-Derived Neurons. PLoS ONE 6(8): e24169. doi:10.1371/journal.pone.0024169 Editor:StevenBarnes,DalhousieUniversity,Canada ReceivedMay6,2011;AcceptedAugust1,2011;PublishedAugust26,2011 Copyright:?2011Risner-Janiczeketal.Thisisanopen-accessarticledistributedunderthetermsoftheCreati