Endo-Lysosomal Vesicles Positive for Rab7 and LAMP1 Are Terminal Vesicles for the Transport of Dextran 英文参考文献.docVIP

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Endo-Lysosomal Vesicles Positive for Rab7 and LAMP1 Are Terminal Vesicles for the Transport of Dextran 英文参考文献.doc

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Endo-Lysosomal Vesicles Positive for Rab7 and LAMP1 Are Terminal Vesicles for the Transport of Dextran 英文参考文献

Endo-LysosomalVesiclesPositiveforRab7andLAMP1 AreTerminalVesiclesfortheTransportofDextran WilliamH.Humphries,IV,CraigJ.Szymanski,ChristineK.Payne* SchoolofChemistryandBiochemistryandPetitInstituteforBioengineeringandBioscience,GeorgiaInstituteofTechnology,Atlanta,Georgia,UnitedStatesofAmerica Abstract The endo-lysosomal pathway is essential for intracellular transport and the degradation of extracellular cargo. The relationship between three populations of endo-lysosomal vesicles—Rab7-positive, LAMP1-positive, and both Rab7- and LAMP1-postive—was probed with fluorescence microscopy and single particle tracking. Of specific interest was determining ifthesevesicles wereintermediate orterminal vesicles inthetransport ofextracellular cargo. Wefindthat the major organelle in the endo-lysosomal pathway, both in terms of population and cargo transport, is positive for Rab7 and LAMP1. Dextran, a fluid phase cargo, shifts from localization within all three populations of vesicles at 30 minutes and1hourtoprimarily LAMP1-andRab7/LAMP1-vesicles atlonger times. Thisdemonstrates thatLAMP1- and Rab7/LAMP1-vesicles are terminal vesicles in the endo-lysosomal pathway. We tested two possible mechanisms for thisdistribution ofcargo,delivery tomannose 6-phosphate receptor (M6PR)-negative vesicles andthefusiondynamics of individual vesicles. We find no correlation with M6PR but do find that Rab7-vesicles undergo significantly fewer fusion events than LAMP1- or Rab7/LAMP1-vesicles suggesting that the distribution of fluid phase cargo is driven by vesicle dynamics. Citation:HumphriesWHIV,SzymanskiCJ,PayneCK(2011)Endo-Lysosomal VesiclesPositiveforRab7andLAMP1AreTerminalVesiclesfortheTransportof Dextran.PLoSONE6(10):e26626.doi:10.1371/journal.pone.0026626 Editor:SteveHCaplan,UniversityofNebraskaMedicalCenter,UnitedStatesofAmerica ReceivedJune28,2011;AcceptedSeptember29,2011;PublishedOctober24,2011 Copyright: ?2011 Humphries etal.Thisisanopen-access article distributedunder th