Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy 英文参考文献.docVIP

Epigenetics Chromatin BioMedCentral Research Open Access Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy Xueying Wang1,3, Zvi Kam2, Peter M Carlton1, Lifeng Xu1, John W Sedat1 and Elizabeth H Blackburn*1 Address: 1Department of Biochemistry and Biophysics, University of California at San Francisco, California, USA, 2Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel and 3Genome Institute of Singapore, Singapore Email: Xueying Wang - wangxs@.sg; Zvi Kam - zvi.kam@weizmann.ac.il; Peter M Carlton - pmcarlton@; Lifeng Xu - lifeng.xu@; John W Sedat - sedat@; Elizabeth H Blackburn* - elizabeth.blackburn@ * Corresponding author Published: 27 October 2008 Received: 28 May 2008 Accepted: 27 October 2008 Epigenetics Chromatin 2008, 1:4 doi:10.1186/1756-8935-1-4 This article is available from: /content/1/1/4 ? 2008 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Telomeres cap chromosome ends and protect the genome. We studied individual telomeres in live human cancer cells. In capturing telomere motions using quantitative imaging to acquire complete high-resolution three-dimensional datasets every second for 200 seconds, telomere dynamics were systematically analyzed. Results: The motility of individual telomeres within the same cancer cell nucleus was widely heterogeneous. One class of internal heterochromatic regions of chromosomes analyzed moved more uniformly and showed less motion and heterogeneity than telomeres. The single telomere analyses in cancer cells revealed that shorter telomeres showed more motion, and the more rapid telomere motions were energy dependent. Experimentally increasing