Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity 英文参考文献.docVIP

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Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity 英文参考文献.doc

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Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity 英文参考文献

SelectionofDNAAptamersagainstGlioblastomaCells withHighAffinityandSpecificity DezhiKang1*,JiangjieWang1,WeiyunZhang2,YanlingSong2,XilanLi2,YuanZou2,MingtaoZhu1, ZhiZhu2,FuyongChen1,ChaoyongJamesYang2* 1Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China, 2State Key Laboratory of Physical Chemistry of Solid Surfaces,KeyLaboratoryforChemicalBiologyofFujianProvince,KeyLaboratoryofAnalyticalScienceandDepartmentofChemicalBiology,CollegeofChemistryand ChemicalEngineering,XiamenUniversity,Xiamen,Fujian,China Abstract Background:Glioblastomaisthemostcommonandmostlethalformofbraintumorinhuman.Unfortunately,thereisstill noeffectivetherapytothisfataldiseaseandthemediansurvivalisgenerallylessthanoneyearfromthetimeofdiagnosis. Discovery of ligands that can bind specifically to this type of tumor cells will be of great significance to develop early molecularimaging,targeteddeliveryandguidedsurgerymethodstobattlethistypeofbraintumor. Methodology/Principal Findings: We discovered two target-specific aptamers named GBM128 and GBM131 against cultured human glioblastoma cell line U118-MG after 30 rounds selection by a method called cell-based Systematic EvolutionofLigandsbyEXponentialenrichment(cell-SELEX).Thesetwoaptamershavehighaffinityandspecificityagainst targetglioblastomacells.Theyneitherrecognizenormalastraglialcells,nordotheyrecognizeothernormalandcancercell linestested.Clinicaltissueswerealsotestedandtheresultsshowedthatthesetwoaptamerscanbindtodifferentclinical gliomatissuesbutnotnormalbraintissues.Moreimportantly,bindingaffinityandselectivityofthesetwoaptamerswere retainedincomplicatedbiologicalenvironment. Conclusion/Significance: The selected aptamers could be used to identify specific glioblastoma biomarkers. Methods of molecularimaging,targeteddrugdelivery,ligandguidedsurgerycanbefurtherdevelopedbasedontheseligandsforearly detection,targetedtherapy,andguidedsurgeryofglioblastomaleadingtoeffectivetreatmentofglio