Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process 英文参考文献.docVIP

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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process 英文参考文献.doc

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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process Marino Expósito-Rodríguez*1,2, Andrés A Borges1, Andrés Borges-Pérez1 and José A Pérez2 Address: 1Instituto de Productos Naturales y Agrobiología – CSIC, Avda. Astrofísico Francisco Sánchez 3, P.O. Box 195, 38206 La Laguna, Tenerife, Canary Islands, Spain and 2Departamento de Parasitología, Ecología y Genética – Facultad de Biología, Universidad de La Laguna, Avda. Astrofísico Francisco Sánchez s/n, 38271 La Laguna, Tenerife, Canary Islands, Spain Email: Marino Expósito-Rodríguez* - marino@ipna.csic.es; Andrés A Borges - aborges@ipna.csic.es; Andrés Borges- Pérez - andborg@ipna.csic.es; José A Pérez - joanpere@ull.es * Corresponding author Published: 22 December 2008 Received: 4 August 2008 Accepted: 22 December 2008 BMC Plant Biology 2008, 8:131 doi:10.1186/1471-2229-8-131 This article is available from: /1471-2229/8/131 ? 2008 Expósito-Rodríguez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The elucidation of gene expression patterns leads to a better understanding of biological processes. Real-time quantitative RT-PCR has become the standard method for in-depth studies of gene expression. A biologically meaningful reporting of target mRNA quantities requires accurate and reliable normalization in order to identify real gene-specific variation. The purpose of normalization is to control several variables such as different amounts and quality of starting material, variable enzymatic efficiencies of retrotranscription from RNA to cDNA, or differences between tissues or cells in overall transcriptiona