Selective Cytotoxicity of Goniothalamin against Hepatoblastoma HepG2 Cells 英文参考文献.docVIP

Molecules 2011, 16, 2944-2959; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Selective Cytotoxicity of Goniothalamin against Hepatoblastoma HepG2 Cells Mothanna Al-Qubaisi 1, Rosli Rozita 2, Swee-Keong Yeap 1, Abdul-Rahman Omar 3, Abdul-Manaf Ali 4 and Noorjahan B. Alitheen 1,* 1 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia 2 Department of Obstetric and Gynaecology, Faculty of Medicine and Health Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia 3 Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia 4 Faculty of Agriculture and Biotechnology, University Sultan Zainal Abidin (UniSZA), Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia * Author to whom correspondence should be addressed; E-Mails: noorjahan@.my or noorjahan@; Tel.: +603-8946 7471; Fax: +603-8946 7510. Received: 17 January 2011; in revised form: 11 March 2011 / Accepted: 17 March 2011 / Published: 6 April 2011 Abstract: Liver cancer has become one of the major types of cancer with high mortality and liver cancer is not responsive to the current cytotoxic agents used in chemotherapy. The purpose of this study was to examine the in vitro cytotoxicity of goniothalamin on human hepatoblastoma HepG2 cells and normal liver Chang cells. The cytotoxicity of goniothalamin against HepG2 and liver Chang cell was tested using MTT cell viability assay, LDH leakage assay, cell cycle flow cytometry PI analysis, BrdU proliferation ELISA assay and trypan blue dye exclusion assay. Goniothalamin selectively inhibited HepG2 cells [IC50 = 4.6 (±0.23) μM in the MTT assay; IC50 = 5.20 (±0.01) μM for LDH assay at 72 hours], with less sensitivity in Cha