Seroconversion to HCoV-NL63 in Rhesus Macaques 英文参考文献.docVIP

Viruses 2009, 1, 647-656; doi:10.3390/v1030647 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Communication Seroconversion to HCoV-NL63 in Rhesus Macaques Ronald Dijkman 1, H. Lie Mulder 1, Lynne Rumping 1, Ilse Kraaijvanger 1, Martin Deijs 1, Maarten F. Jebbink 1, Ernst J. Verschoor 2 and Lia van der Hoek 1,* 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity (CINIMA), Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands; E-Mails: r.dijkman@amc.uva.nl (R.D.); h.l.mulder.1@student.rug.nl (H.L.M.); lynne.rumping@student.uva.nl (L.R.); ilse.kraaijvanger@student.uva.nl (I. K.); m.deijs@amc.uva.nl (M.D.); m.f.jebbink@amc.uva.nl (M.F.J.) 2 Department of Virology, Biomedical Primate Research Centre (BPRC), Lange Kleiweg 139, 2280 GH, Rijswijk, The Netherlands; E-Mail: verschoor@bprc.nl (E.J.V.) * Author to whom correspondence should be addressed; E-Mail: c.m.vanderhoek@amc.uva.nl; Tel.: +31-20-5667510; Fax: +31-20-6916531. Received: 26 August 2009; in revised form: 28 October 2009 / Accepted: 30 October 2009 / Published: 30 October 2009 Abstract: HCoV-NL63 is a recently identified respiratory virus. Its pathogenesis has not been fully unraveled because an animal model is currently lacking. Here we examined whether rhesus macaques encounter HCoV-NL63 infections during life, by examining the levels of antibodies to HCoV-NL63 in time. The animals were followed for 7 up till 19 years, and in three animals we observed a steep rise in antibodies during follow up, indicative of a natural infection with HCoV-NL63. Keywords: HCoV-NL63; rhesus macaques 1. Introduction HCoV-NL63 was identified in 2004 in a child with bronchiolitis in The Netherlands [1], yet worldwide screenings revealed that the virus can be detected throughout the globe in children and adults with respiratory tract infect