Statistical methods for ranking differentially expressed genes 英文参考文献.docVIP

Open Access d 2003BrobergVolumeMetho4, Issue 6, Article R41 Statistical methods for ranking differentially expressed genes Per Broberg Address: Molecular Sciences, AstraZeneca Research and Development Lund, S-221 87 Lund, Sweden. Correspondence: Per Broberg. E-mail: per.broberg@. Published: 29 May 2003 Received: 9 December 2002 Revised: 25 March 2003 Accepted: 7 May 2003 Genome Biology 2003, 4:R41 The electronic version of this article is the complete one and can be found online at /2003/4/6/R41 A previous version of this manuscript was made available before peer review at /2002/3/9/preprint/0007 ? 2003 Broberg; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. StatisticalItloienfssttttshhsteethamaatnitesagtlhimethodsycvosewdisf.ietohwf mwfahiforlciscreohprankingatororsaritayivdkeastgdifferentiallyaaenthdesfeiwdwietnhfatrisfeiescexpressedpaneteicogtanttooivdfedisfif.genesfferentialeErsetnimtiaalteioxexpressionnproefstshioenf.aTlisseesparamount.-tnseogfatthiveemaestHerewhoeldl asIhsoutlineothwetfhaaltsaeit-methodpaollsoiwtisvegforernatefindingeraliteiosnatoantfhtoptimalephegeneart Abstract In the analysis of microarray data the identification of differential expression is paramount. Here I outline a method for finding an optimal test statistic with which to rank genes with respect to differential expression. Tests of the method show that it allows generation of top gene lists that give few false positives and few false negatives. Estimation of the false-negative as well as the false- positive rate lies at the heart of the method. Background impact on decisions regarding target selection by providing a preliminary ranking of the genes. This is not to say that mul- tiplicity should never be taken into account, or that the meth- od presented here replaces correcti