Translation reinitiation and development are compromised in similar ways by mutations in translation initiation factor eIF3h and the ribosomal protein RPL24 英文参考文献.docVIP

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Translation reinitiation and development are compromised in similar ways by mutations in translation initiation factor eIF3h and the ribosomal protein RPL24 英文参考文献.doc

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Translation reinitiation and development are compromised in similar ways by mutations in translation initiation factor eIF3h and the ribosomal protein RPL24 英文参考文献

Zhouetal.BMCPlantBiology2010,10:193 /1471-2229/10/193 RESEARCH ARTICLE OpenAccess Translationreinitiationanddevelopmentare compromisedinsimilarwaysbymutationsin translationinitiationfactoreIF3handthe ribosomalproteinRPL24 FujunZhou1,3,BijoyitaRoy2,AlbrechtGvonArnim2* Abstract Background:Withinthescanningmodeloftranslationinitiation,reinitiationisanon-canonicalmechanismthat operatesonmRNAsharboringupstreamopenreadingframes.Thehsubunitofeukaryoticinitiationfactor3(eIF3) booststranslationreinitiationontheuORF-containingmRNAcodingfortheArabidopsisbZiptranscriptionfactor, AtbZip11,amongothers.TheRPL24Bproteinofthelargeribosomalsubunit,whichisencodedbySHORTVALVE1, likewisefosterstranslationofuORF-containingmRNAs,forexamplemRNAsforauxinresponsetranscriptionfactors (ARFs). Results:HerewetestedthehypothesisthatRPL24BandeIF3haffecttranslationreinitiationinasimilarfashion. First,likeeif3hmutants,rpl24bmutantsunder-translatetheAtbZip11mRNA,andthedetailedspectrumof translationaldefectsinrpl24bisremarkablysimilartothatofeif3h.Second,eif3hmutantsdisplaydefectsinauxin mediatedorganogenesisandgeneexpression,similartorpl24b.LikeAtbZip11,theuORF-containingARFmRNAs areindeedundertranslatedineif3hmutantseedlings. Conclusion:Weconcludethat,similartoeIF3h,RPL24BbolstersthereinitiationcompetenceofuORF-translating ribosomes.CoordinationbetweeneIF3andthelargeribosomalsubunithelpstofine-tunetranslationofuORF- containingmRNAsand,inturn,toorchestrateplantdevelopment. Background good as soon as it has terminated translation at a stop Ineukaryotic cells, geneexpression ishighly regulated, codon. However, there are exceptions to this rule. In often at multiple levels, such as transcription, mRNA Arabidopsis, about 30% of full-length mRNAs harbor structureandstability,translationalcontrol,andprotein oneormoreupstreamopenreadingframes(uORFs)in degradation. Translational regulation is arguably least their 5′ leader sequence [8]. Once a uORF has been well characterized, and questions conce