Variation of Oleanolic and Ursolic Acid in the Flesh of？Persimmon Fruit among Different Cultivars 英文参考文献.docVIP

Molecules 2010, 15, 6580-6587; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Variation of Oleanolic and Ursolic Acid in the Flesh of Persimmon Fruit among Different Cultivars Chunhua Zhou 1, Yanle Sheng 1, Daqiu Zhao 1, Zhiqin Wang 2 and Jun Tao 1,* 1 College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; E-Mails: chzhou@ (C.Z.); ylsheng2007@163.com (Y.S.); 405323488@ (D.Z.) 2 College of Agronomy, Yangzhou University, Yangzhou 225009, China; E-Mail: zqwang@ (Z.P.) * Author to whom correspondence should be addressed; E-Mail: taojun@; Tel.: +86 514 Fax: +86 514 Received: 5 August 2010; in revised form: 25 August 2010 / Accepted: 1 September 2010 / Published: 20 September 2010 Abstract: Oleanolic acid (OA) and ursolic acid (UA) are important bioactive components in many plants, including persimmon (Diospyros kaki L.). The present work was carried out to determine OA and UA contents in the flesh of persimmon fruit from 32 cultivars, including 23 astringent and 9 non-astringent ones, by applying high performance liquid chromatography (HPLC) with UV detection. Both OA and UA were present in all of the investigated cultivars, except for three, ‘Hiratanenashi’, ‘Ribenhongshi’ and ‘Matsumotowase’. The OA content ranged from traces to 88.57 μg/g FW, and that of UA were between traces and 27.64 μg/g FW. Keywords: persimmon; oleanolic acid; ursolic acid; pentacyclic triterpenoid; HPLC 1. Introduction Persimmon (Diospyros kaki L., Ebenaceae) is a major horticultural crop of China, and is widely cultivated in other parts of Northeast Asia, including Korea, and Japan [1]. Persimmon fruits are rich in different kinds of nutrients and phytochemicals such as carbohydrates, organic acid, vitamins, tannins, polyphenols, dietary fiber, triterpenoids, and carotenoids, which contribute significantly to