Variation of Cholinesterase-Based Biosensor Sensitivity to Inhibition by Organophosphate Due To Ionizing Radiation 英文参考文献.docVIP

Sensors 2009, 9, 5580-5589; doi:10.3390/OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Variation of Cholinesterase-Based Biosensor Sensitivity to Inhibition by Organophosphate Due To Ionizing Radiation Miroslav Pohanka 1,* and Miroslav Koch 2 1 Centre for Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50002 Hradec Kralove, Czech Republic 2 VF Company, Namesti Miru 50, 679 21 Cerna Hora, Czech Republic; E-Mail: miroslav.koch@vf.cz * Author to whom correspondence should be addressed; E-Mail: rau@atlas.cz; pohanka@pmfhk.cz; Tel.: +420-973-251-519; Fax: +420-495-518-094 Received: 22 May 2009; in revised form: 27 June 2009 / Accepted: 1 July 2009 / Published: 14 July 2009 Abstract: A cholinesterase based biosensor was constructed in order to assess the effects of ionizing radiation on exposed AChE. Although the primary objective of the experiment was to investigate the effect of ionizing radiation on the activity of the biosensor, no changes in cholinesterase activity were observed. Current provided by oxidation of thiocholine previously created from acetylthiocholine by enzyme catalyzed reaction was in a range 395–455 nA. No significant influence of radiation on AChE activity was found, despite the current variation. However, a surprising phenomenon was observed when a model organophosphate paraoxon was assayed. Irradiated biosensors seem to be more susceptible to the inhibitory effects of paraoxon. Control biosensors provided a 94 ± 5 nA current after exposure to 1 ppm paraoxon. The biosensors irradiated by a 5 kGy radiation dose and exposed to paraoxon provided a current of 49 ± 6 nA. Irradiation by doses ranging from 5 mGy to 100 kGy were investigated and the mentioned effect was confirmed at doses above 50 Gy. After the first promising experiments, biosensors irradiated by 5 kG