组织纤溶酶原激活剂突变体(La-tPA)转基因鼠的建立-Al2O3.PDF

31 生命科学研究 Vol.3 No. 1 1999 3 Life Science Research Mar. 1999 : 1007-7847( 1999) 01-0075-04 (La-tPA) X 卢一凡,　邓继先,　程　萱,　周　江,　黄培堂 ( , 100071) 3 3 :用羊 -乳球蛋白基因( ) 5ø区5×10 ( 10 , 旧 ) 为调控序列, B LG b b kb 构建了乳腺表达组织纤溶酶原激活剂突变体( La-tPA) 载体. 对540枚小鼠受精 卵进行显微注射, 经PCR 和Southern blot 检测, 获得6只整合有人La-tPA 的转 基因小鼠, 整合率为32%. 这为未来利用转基因动物生产La-tPA 提供依据. : - ; 内含子;乳腺; 转基因鼠 La tPA : Q75; R394　　　:A Establishment of Transgenic Mice of La-tPA in Mammary Gland Expression LU Yi-fan, DENG Ji-xian, CHENG Xuan, HOU , UANG - Z Jiang H Pei tang ( Institute of itechnique Academy of Military Medical Science eijing, , 100071,China) : - Abstract T he vector of expression La tPA in mammary gland of transgenic animal was constructed by using LG gens 5øcontrol region.6 transgenic mice were produced by mi- croinjection method Foreign gene intergation rate was. 32% in mice It provided us some. information and methods to produce foreign protein in livestocks. : - ; ; ; Key words La tPA intron mammary transgenic mice [ 1] Gordon (tPA) , , . ., . X : 1998-12-07 : ( 101-05-04-01) : ( 1963-) , , , , 20, : ( 010) - : @ T el E mail L u Yifan hotmail com; ( 1956-) , , , ; ( ) . 1999 76