《土壤生物学》中英论文.docxVIP

　Plant, Cell Environment 植物，细胞与环境 Difference in the distribution and speciation of cellular nickel between nickel-tolerant and non-tolerant?Nicotiana tabacum?L. cv. BY-2 cells 在镍耐受和非耐受烟草品种的种子中的BY-2细胞系细胞的镍的分布和形态的区别。 ABSTRACT To evaluate Ni dynamics at the subcellular level, the distribution and speciation of Ni were determined in wild-type (WT) and Ni-tolerant (NIT) tobacco BY-2 cell lines. When exposed to low but toxic levels of Ni, NIT cells were found to contain 2.5-fold more Ni (14% of whole-cell Ni values) in their cell walls than WT cells (6% of whole-cell Ni values). In addition to higher levels of Ni in the apoplast, a higher proportion (94%) of symplastic Ni was localized in the vacuoles of NIT cells than in the vacuoles of WT cells (81%). The concentration of cytosolic Ni in the NIT cells was significantly lower (18?nmol?g?1?FW) than that in the WT cells (85?nmol?g?1?FW).?In silico?simulation showed that 95% of vacuolar Ni was in the form of Ni-citrate complexes, and that free Ni2+?was virtually absent in the NIT cells. On the other hand, the amount of free metal ions was markedly increased in WT cells because free citrate was depleted by chelation of Ni. A protoplast viability assay using BCECF-AM further demonstrated that the main mechanism that confers strong Ni tolerance was present in the symplast as opposed to the cell wall. 摘要 为了在亚细胞水平评估镍动力学，在野生型（WT）和Ni容错（NIT）烟草BY-2细胞系中对镍的分布和形态进行了测定。结果显示，镍的水平低，但毒性水平，没有找到的NIT细胞包含在它们的细胞壁比野生型细胞的2.5倍以上的Ni（全细胞的Ni值的14％）（全细胞的Ni值的6％）。除了在质外体更高水平的Ni，在共质体的Ni的比例上，NIT细胞的空泡较高（94％）定位于野生型细胞（81％）的空泡。胞质的Ni在NIT细胞浓度显著降低（18纳摩尔克-1 FW），但高于野生型细胞（85纳摩尔克-1 FW）。在电脑模拟表明，95％的液泡的Ni是镍柠檬酸复合物的形式，以及游离Ni2?? +的在NIT细胞几乎不存在。另一方面，游离的金属离子的量在野生型细胞中显着增加，因为游离柠檬酸耗尽了镍。用BCECF-AM原生质体活力测定进一步证明了赋予镍强耐受的主要机制是存在的共质体，而不是细胞壁。 INTRODUCTION Nickel (Ni) – an essential micronutrient in plants – participates in nitrogen metabolism as a cofactor of urease, which catalyses the decomposition of urea to ammonium and carbon dioxide ( HYPERLINK /doi/10.1111/j.1365-3040.200