虎眼万年青提取物对肝纤维化治疗作用探讨.docVIP

虎眼万年青提取物对肝纤维化的治疗作用研究 　　[摘要] 采用四氯化碳（CCl4）制备小鼠肝纤维化模型，检测AST，ALT，γGT，MDA，GSHpx，SOD等血清指标，ELISA方法检测PCⅢ，IVC，LN，HA等血清标记物；肝组织采用HE和Masson胶原染色，光镜下观察病理学改变；Western blot检测Ⅲ型胶原、TGFβ，αSMA，Ecadherin蛋白表达，观察虎眼万年青提取物对CCl4诱导的小鼠肝纤维化治疗作用，并探讨作用机制。结果表明，虎眼万年青提取物组（50，150，500 mgkg-1）均可明显降低血清AST，ALT，γGT，MDA水平，升高GSHpx，SOD水平；降低肝纤维化血清标志物 PCⅢ，IVC，LN，HA的表达；明显改善CCl4所致大鼠肝纤维化的病理学改变，证明虎眼万年青提取物对肝纤维化具有治疗作用。其机制是可能与抑制细胞外基质沉积，抑制肝星形细胞活化以及抑制上皮间质转化，进而抑制肝脏纤维化有关 [关键词] 虎眼万年青； 肝纤维化 Research ontherapeutics effect of extract of Ornithogalum caudatum on liver fibrosis GAO Lu1， ZHANG Ming2， SU Ting1， QU Jiale1， JIANG Wenyue3* （1Jilin Modern Chinese Medicine Engineering and Research Center Co， Ltd， Changchun 130012， China； 2School of Basic Medical Science， Jilin University， Changchun 130000， China； 3Changchun Kang Bida Technology Co， Ltd， Changchun 130012， China） [Abstracr] Rat models of liver fibrosis were made by carbon tetrachloride， and the serum levels of AST， ALT， γGT， MDA， GSHpx， SOD were detected， serum markers of PCⅢ， IVC， LN， HA were detected by ELISA method HE and Masson staining were conducted in hepatic tissues to observe pathological variations Collagen Ⅲ， TGFβ， αSMA， Ecadherin were detected by Western blot The curative effect of the extract of Ornithogalum caudatum on rat liver fibrosis induced by CCl4was observed and the mechanism was discussed The experiment results showed that the extract of O caudatum （50， 150， 500 mgkg-1） obviously decreased the serum levels of AST， ALT， γGT， MDA， increased the serum levels of GSHpx， SOD， decreased the expression of serum markers of PCⅢ， IVC， LN， HA， and improved the liver pathological variations of fibrotic rats The experiment proved that the extract of O caudatum could treat the liver fibrogenesis induced by CCl4 in rats The positive medicine may inhibit accumulation of extracellular and activate hepatic stellate cell and epithelialmesenchymal transition [Key words] Ornithogalum caudatum； liver fibrosis doi：10.4268/cjcm 肝纤维化