氧化石墨烯对蛋白质和DNA的构象和功能的影响.pdfVIP

GO sheets intercalated into the planes between the base pairs of the DNA. The intercalation induced the alteration of secondary structure of ctDNA, weakening the π–π stacking interaction between its base pairs and loosening its original contractive structure, but did not lead to the unwinding of its double helical structure. Fluorescence spectra analysis also showed that the exogenous fluorescence of ctDNA was obviously quenched by GO, and the quenching effect was due to a static quenching mechanism. In addition, the binding constant between GO and ctDNA was calculated based on the absorption spectral data. RT-qPCR experiments indicated that GO caused clear changes in amplification and melting curves of DNA in a RT-qPCR, reducing the PCR amplification efficiency, inhibiting the PCR reaction and resulting in the occurrence of the non-specific amplification reaction. The results showed that GO causes the change of secondary conformation of DNA, and produces certain inhibition of the PCR reaction (to some extent DNA replication). These studies demonstrated that a certain concentration of GO could cause negative effects on conformation and function of proteins and DNA, and its applications in biological systems need to be treated with caution and further evaluated. KEY WORDS ：Graphene oxide, Catalase, DNA, Spectroscopy, Conformation, Function 目 录 前 言 1 第一章 文献综述 2 1.1 氧化石墨烯概述 2 1.1.1 氧化石墨烯的结构与特性 2 1.1.2 氧化石墨烯的应用 3 1.2 氧化石墨烯的生物安全性 5 1.3 蛋白质和DNA 的光谱学研究概况 6 1.3.1 紫外–可见吸收光谱法 6 1.3.2 荧光光谱法 7 1.3.3 圆二色光谱法 8 1.3.4 傅里叶变换红外光谱法 9 1.4 本文的研究意义和主要内容 10 第二章 氧化石墨烯对过氧化氢酶的构象和活性的影响 11 2.1 材料与仪器 11