湿阻中焦证ICC模型的建立及平胃散的干预作用.pdfVIP

Result： 1.The succeed build the model of the vitro dampness obstructing spleen-stomach syndrome in ICC: ①By morphological abserve, syndrome cell model morphology has changed significantly: Cells become flat, Cells become flat, cell gap become smaller , Network connection is not obvious; ②Objective indicators for detection of syndrome cell model compared with normal cells, cell viability、metabolic、signal transduction are changed: Intracellular ATP of syndrome cell model increased, Na+-K+-ATP enzyme activity decreased, Intracellular K+ concentration increased, cell anaerobic metabolism increased, Inositol triphosphate (IP3) levels tended to increase. Description of normal cells after modeled , there are some changes in cell metabolism, ③After Pingwei Powder treatment to the model cell , cell viability and metabolic are changed: Compared with normal cells, the dampness obstructing spleen-stomach syndrome of Pingwei powders serum model can decrease syndrome cell model intracellular ATP, Cell Na+-K+-ATP enzyme activity increased , Pingwei Powder can reduce dampness obstructing spleen-stomach syndrome in ICC intracellular LDH activity 、inhibit syndrome ICC anaerobic metabolism、Promote intracellular second messenger IP3 content，It can be speculated that dampness obstructing spleen-stomach syndrome in ICC model is successful 2. Pingwei Powderdifferent administration impact on the ICC: ①Pingwei Powder direct administration to the normal ICC: The direct administration of different concentrations of Pingwei Powder prompted cell ATP decreased, Cell Na+-K+-ATP enzyme activity increased, The intracellular K+concentration reduced, Intracellular IP3 increased, Intracellular LDH activity was