重庆彭水野生美味牛肝菌菌丝分离与鉴定.pdfVIP

中国食用商 2012 , 31 (1): 46-48 CN53-1054/Q ISSN 1∞3-8310 ED1 BLE FUNGI OF CHINA 重庆彭水野生美昧牛肝菌菌丝分离与鉴定* 王正春 I 气蒋海艳1 ，杨笑笑2 ，李月文 1 ，李继晖 3 ，郑凌凌3 (1.重庆市林业科学研究院，重庆 4α浏览; 2.西南大学资源环境学院，重庆 4∞715; 3.彭水自治县林业科技推广站，四川彭水 4096∞) 摘要:通过2 种不同的培养基对彭水2 种野生牛肝菌子实体进行分离、培养，同时，进行液体培养，培养的菌丝经过 口s鉴定，放入-80C超低温冰箱中保藏。结果表明， 2 种牛肝茵茵丝均从组织块表面边缘生长，茵丝洁白、小，不向周 围培养基生长，老化速度较快，老化过程中菌丝减少，最后变为团体状物，并且分泌褐色水珠;液体培养菌丝体生长 缓慢，培养时间较长，菌丝球小、少;茵丝球经过鉴定后，确定2 种牛肝菌均为美味牛肝菌。 关键词:重庆;彭水;美味牛肝菌;分离培养;液体培养; ITS 鉴定;保藏 中图分类号: S646.9 文献标识码:A 文章编号: 1003-8310 (2012) 01-(附46-03 Hyphe Separation and Identification of Wild Boletus edulis in Chongqing Pengshui 2 WANG Zheng-chunt.2, JIANG Hai-yan飞 YANG Xiao-xiao , LI Yue-wen, LI Ji-hui气 ZHENG Ling-li吨3 (I.Forestry Research Institute of Chongqin臣， Chongqin 4α)()36; 2.Southwest University , Chongqin 4∞715; 3.Pengshui Autonomous County Forestry Science and Technology Promotion Station, Pengshui Sichuan 409创页。) Abstract: Through two different culture medium , two wild Bolet u.s edu1is were seperated and cultured, and training hy- phae were identificated by 1 15, which were stord in the refrigerator of -80C. The results showed that the two Bolet u.s edu1is hyphe liver tissue were growth from mass surface edge, and the hyphe we陀 white and small. The hyphe didn t grow around.the culture medium , and aging faster. The mycelia by liquid culture grew slowly, and the ball was small. Hyphae ball by determining we陀 Boletω edu1is. Key words: Chongqing; Pengshui; Bolet u.s edu1is; Separation training; Liquid culture; 115 identification; Preservation