茶多糖提取方法及影响因子优化研究.pdfVIP

, Jou rn al o A nhu iA gr.i Sci. 2010, 38( 16) : 8650 - 8652 刘月娟 况玲玲 刘 悦, 徐东升, 臧其威 ( , 221116 ) [目的]找出茶多糖的最优提取方法和对提取率的影响因子[ 方法] 测定采 醇沉淀法CTAB 沉淀法微波水提法 3种提取方 法的提取率研究提取时间液固比提取温度和提取次数 4 个因素对茶叶中茶多糖提取率的影响, 并利 正交试验进行优化[ 结果] 较优的提取条件为: 提取时间60 m in, 蒸馏水与茶叶的液固比 301, 浸提温度 60 ~ 70 , 提取次数 3次, 茶多糖的提取率达 2120% [结论]采 醇沉淀法提取茶多糖时效果最优, 浸提时间液固比浸提温度和浸提次数均与提取率呈正相关 茶多糖; 提取方法; 影响因子; 优化条件 TS 272 A 0517 - 6611( 2010) 16- 08650- 03 Study on ExtractionMethods ofTeaPolysaccharides from Tea andOptimum Extraction Conditions LIU Yue et al ( School o Ch em ica lE ng ineering T echno logy, Ch ina U n iversity o M in ing T echno logy, Xu zhou, J iang su 221116) Abstract [ Objective] The research a mi ed to in d th e best ex traction m ethod and the e ect actors o th e extraction yield. [M eth od] Th e ex- traction y ield o tea po lysacch aridesw as com pared by the m e thod o u sing e thano,l the m ethod o u sing CTAB, and th e m ethod o using m icro- w ave. Extraction process w as stud ied rom our actors in clud ing ex tracting tmi e, the ratio o liqu id to so lid, ex tracting tem p erature, and ex- tracting tmi es in th e m ethod o ethano.l [ Result] The optmi um extraction cond ition w as as o llow s: ex tracting tmi e o 60 m in, ratio o liqu id to solid o 301, ex tracting tem p erature o 60 - 70 , extracting tmi es o 3. And th e ex traction y ield o tea po lysaccharides w as 2. 120% [ Con clu sion] Th e best m ethod w as u sing eth anol, and th e e ect actorsw ere related to th e extraction y ield. eywords T ea p olysacch arides; Ex traction m eth od; E ect actors; Optmi um conditions (TPS) , ( 2, 3 m in /), ( 2 ), , , , ( 95% ), , , 2, , , [ 1] , , , , 2 [ 6]