HBV分子生物学-培训课件.pptVIP

复习题 简述HBV基因组特征． 简述HBV复制过程以及可能的阻断病毒复制的位点． 简述HBV治疗药物种类和应用中存在问题． * (A) Maps with imposed icosahedral symmetry arising from the same virion data set but different starting models yielded variable yet comparably spaced surface features. Surface-shaded views of three representative maps (out of eight) viewed along the 2-fold (2F), 5-fold (5F), and 3-fold (3F) symmetry axes. Map 1, the same as shown in Figure 3C; map 2, result from the final map from refinement of density within radii spanning the capsid used as the starting model (Figure 3A); and map 3, result from a starting model generated by applying random orientations to the particles. (B) Despite completely different starting models, each map displayed similar size and packing of the surface projections but had more poorly defined density at varying icosahedral symmetry positions. This pattern may arise if the projections have ordered packing with random insertion of pentamers. Packing of the surface projections is emphasized by the overlay of yellow dots. The red triangle has 60 ? edges and shows the average spacing of the projections. Five-fold and 3-fold axes are indicated by black pentagons and triangles, respectively. (C) Analysis of en face images of HBV. Central, en face views of the T=4 capsid X-ray structure (a), cytoplasmic capsids (b), and virions (c, d, and e) were classified by factor analysis. For all the particle types, class averages (left column) displayed discrete densities, which gave rise to sampled reflections in the Fourier transforms (FFT) (middle column). Circularly averaged, 1D profiles of the FFTs (right column) displayed broad peaks. The capsid X-ray structure (a) and the cytoplasmic capsids (b) displayed very similar profiles. A peak at 1/43 ? corresponded to the separation between adjacent spikes and a smaller peak at 1/80 ? was interpreted as diffraction between the second nearest neighbor of spikes. The virion images yielded three classes with different profiles. Class I showed a pea