利用响应面法优化鼠李糖乳杆菌TUST006冷冻干燥保护剂.pdfVIP

现代食品科技 ModernFoodScienceand 2010，V01．26，No．9 Technology 利用响应面法优化鼠李糖乳杆菌 TUST006冷冻干燥保护剂 刘会平，马丽，戴广丽，闫清泉，徐乙文 (天津科技大学食品工程与生物技术学院，天津300457) 摘要：本文用2水平7因子和响应面分析法筛选优化了鼠李糖乳杆菌TUST006冷冻干燥的保护剂配方．结果表明，脱脂乳、海 藻糖，谷氨酸钠能显著提高细胞对冷冻和干燥的环境耐受能力．以冻干后的细胞存活率为响应值，混合保护剂的最佳配方为脱脂乳 8．62％，海藻糖2．78％，谷氨酸钠6．74％，细胞存活率为96．44％，贮存两个月后细胞存活率可保持在90．30％． 关键词：鼠李糖乳杆菌TUST006；冷冻干燥保护剂；二水平部分因子设计；响应面法 文章篇号：1673．9078(2010)9．975-978 of forLactobacillusrhamnosusTUST006 OprimizationCryoprotectants by SurfaceMethods Response LIU Yi-wen Hui-ping,MALi,DaiGuang-li,YANQing-quan，xu ofFood and ofScienceand (CollegeEngineeringBiotechnology，TianjinUniversity Technology,Tianjin300457，China) Abstract：Theformulaof forLactobacillusrhamnosusTUST006was 2-levelfactorial andBox by design cryoproteetants optimized Behnkcn resultsindicatedthattheskim trehaloseand80diunl arethree contributetothe milk factors，which Design(BBD)．The glutamatekey survivalcellsafter BBD and surfaoe modelforthethree factorsWas ofthe cryodesieeation．Bydesignresponseanalysis，thequadratic significant rate skimmilk sodium establishedwithcellsurvivalasthe formulaisobtained鹊：the8．62％．1∞ohaloso2．78％and targetresponse．Theoptima whichthecellsurvivalratereaches thecellsurvivalrateCan 90．30％after2-month 6．74％，with 96．44％，and preserves storage． glutamate