全自动阅片机测定血小板计数方法的可行性研究.pdfVIP

• 37 • l临床输血与检验 2015 年 2 月第 17 卷第 1 期 全自动阅片机测定血小板计数方法的可行性研究 洪骏戴雯王静时永挥 E摘要】 目的 探讨CELLDIFF BCM-2 全自动阅片机测定血小板(PLT)计数与Sysmex XE-5000 全自动血细胞分析 仪、子 r 计数法检测结果的可比性。方法 随机选择300 例抗凝全血，首先采用全自动血细胞分析仪阻抗法测定PLT 值，按 PLT 数值将其分为低、中、高3 组，再通过全自动阅片机计数法分别检测3 组标本的PLT 值，同时与血细胞分析仪光学法的 测定结果进行比较，最后以子工法作为参考方法对上述方法的PLT 计数结果进而初步评价。结果 在低、中、高PLT 组中， 阅片机计数法检测的PLT 值与光学法的计数结果的差异元统计学意义(PO.O日，但与阻抗法的计数结果相比，仅在中 PLT 组的差异无统计学意义(PO. 05); 所有标本采用阅片计数法检测的PLT 值与阻抗法(r=O. 989 ,P0. 001) 、光学法 (r=O. 992 ,P0. 001) 、子工法Cr= 0.986 ,PO. 001) 的计数结果均呈显著正相关。阅片计数法测定PLT 可同时观察大PLT 和 PLT 聚集的存在。结论 全自动阅片机测定PLT 计数与全自动血细胞分析仪的阻抗法、光学法及子工法检测结果均具 有高度相关性，能同时观察大PLT 和PLT 聚集现象，具有良好的临床应用价值。 E关键词】全自动阅片机 血小板 复检 E中图分类号】 R33 1. 1+43 (文献标识码】 A (文章编号】 1671-2587(2015)01-0037一04 The Feasibility Study on Automatic Reading Machine in Platelets Counting HONG .Jun ,DAI Wen , WANG .Jing ,et al. In stitute of Lahoratory Medicine Science of P LA ,N anjing Generral Hοs户ital of N anjing Military Command ,P LA ,N anjing 210002 (Abstract Objective To compare the accuracy of platelets counts detected by automatic reading machine ,the Sys mex XE 5000 automated hematology analyzer ,and the manual counting. Methods Three hundreds of samples to be tested for platelet counts were detected by the resistance method , optical method ,automatic reading method , and manual method was used as reference. PLT counts were divided into low , medium ,and high groups ,and the correlation was analyzed a mong the 3 methods. Results There was no significant difference of reading method with the optical method CPO. 05); no significant difference was noted in the medium group and the resistance method. The correlative coefficients of the read ing method were 0.989 ,0.992