关于比色测定法中标准曲线制作的思考.pdfVIP

第 18卷 第 5 期 2012 年 5 月 Vol.18 No.5 Guiding Journal of Traditional Chinese Medicine and Pharmacy May.2012 关于比色测定法中标准曲线制作的思考* 杨泽民，罗少洪，李 明 （广东药学院基础学院，广东 广州 510006 ） [摘要] 目的：探讨比色测定法中标准曲线的制作是否需要过原点。方法：以Folin 酚试剂法测定蛋白质含量为例，通过制作 过原点和不过原点的标准曲线 ，分别计算设定浓度的蛋白质样品的测定值，比较测定值和真实值。结果：不过原点的标准曲线的线 性相关系数R2和由此计算出的测定值都优于过原点的标准曲线。结论：比色测定法中标准曲线的制作不一定要过原点。 [关键词] 比色测定法；标准曲线；原点 [中图分类号] R284.1 [文献标识码] 文章编号 （ ） A [ ] 1672 951X 2012 05 0078 02 Reflections on the colorimetric determination of the standard curve produced in France Yang Zemin, Luo Shaohong, Li Ming (Foundation Institute of Guangdong Pharmaceutical University, Guangzhou Guangdong 510006) [Abstract] Objective: To investigate the colorimetric determination method in the standard curve of production need to square one. Methods: Folin phenol reagent method determination of protein content, for example, produced through the origin but the origin of the standard curve were calculated to set the concentration of protein samples measured values, and compare the measured value and true value. Results: However, the origin of the standard linear correlation coefficient R2 and thus cal culate the measured values are superior to the origin of the standard curve. Conclusions: The colorimetric determination method in the standard curve of production not necessarily through the origin. [Key words] Colorimetric determination method; Standard curve; Origin 比色测定法，又称分光光度法，它是测定生化物质含量 直存在争议。一部分教师认为，郎伯 比尔定律是比色测定法 的常用方法，也