血VEGF水平与上皮性卵巢癌组织微血管生成的关系.pdfVIP

Journal of Medical Forum Vo1．28 No．23 December 2007 · 21· 血VEGF水平与上皮性卵巢癌组织 微血管生成的关系 韩云志，游言文，陈四清，刘风花 ，田新红，王玎玎，郝 莉，程秀娟 河南中医学院解剖组胚学科 郑州市 450008 摘要 目的 探讨上皮性卵巢癌患者血管内皮生长因子(VEGF)水平与癌组织血管生成及卵巢上皮性肿 瘤发展的关系。方法 采用酶联免疫吸附试验双抗体夹心法(ELISA法)检测20例上皮性卵巢肿瘤、12例交界 性上皮性卵巢肿瘤和48例上皮性卵巢癌患者血中VEGF水平，同时以CD34标记肿瘤微血管内皮细胞，应用免 疫组织化学方法检测肿瘤组织中微血管(MVD)的生成密度 结果 良性、交界性及恶性卵巢上皮性肿瘤患者 血VEGF水平依次升高，分别为(686．28±149．68)ng／L、(750．28±90．12)ng／L和(1 183．23±293．22)ng／L (P0．001)，肿瘤组织中微血管(MVD)的生成密度依次升高(P0．001)；上皮性卵巢癌中患者血VGEF与 组织中MVD表达正相关(r=0．625，P0．001)，并在浆液性卵巢腺癌、低分化卵巢腺癌中水平明显升高(P= 0．046、P=0．029)，而与临床分期无关。结论 血VEGF水平与上皮性卵巢癌血管生成相关，可作为反映卵巢 上皮性肿瘤生物学行为的指标之一，有助于判断卵巢癌的预后。 关键词 血管内皮生长因子；微血管密度；卵巢上皮性肿瘤 中图分类号：R737．31 文献标识码：A 文章编号：1672—3422(2007)23—0021-03 Relationship between Serum Vascular Endothelial Growth Factor and Tumor Microvessel Density in Epithelial Ovarian Neoplasm HAN Yunzhi，YOU Yanwan，CHEN Siqing，et al Department of Anatomy，Henan College of Traditional Chinese Medicine， Zhengzhou 450008，China ABSTRACT Objective To investigate the relationship of senlm vascular endothelial growth factor and tumor microvessel density(MVD)in epithelial ovarian neoplasm．Methods Serum levels of VEGF in 48 cases of ovarian carcinoma，1 2 cases of borderline ovarian tumor，20 cases of benign ovarian tumor was measured by Sandwich—ELISA；MVD were counted by manual counting in tumor tissue．Re- suits The Serum levels of VEGF were increased in turn in benign ovarian tumor，borderline ovarian tumor and ovarian carcinoma，P 0．001：MVD were also increased in turn，P 0．O01：There were positive correlation between the serum levels of VEGF and MVD in epithelial ovarian neoplasm．r= 0．625，P 0．00 1，and the serum levels of V