运用O型血孕妇血浆中游离胎儿DNA检测胎儿ABO血型.pdfVIP

现代妲产科进展2007年l2月第l6卷第l2期 Prog Obstet Gynecol，Dec．2007，VoL 16，No．12 · 论著 · 运用O型血孕妇血浆中游离胎儿DNA检测 胎儿ABe血型 付先虎，陈汉平 ，徐晓燕，张宝春 (华中科技大学同济医学院附属同济医院妇产科，武汉 430030) 【摘要】 目的：探讨利用0型血孕妇血浆中游离胎儿DNA检测胎儿ABe血型的可行性。 方法：用酚／氯仿法从39例O型血孕妇(孕36～40”周)且孕男性胎儿的血浆中提取胎儿 DNA，巢式PCR扩增血浆中的SRY基因证实胎儿DNA存在，再用特异性序列一聚合酶链反应 (SSP—PCR)检测胎儿ABe血型。用常规血清学方法测母体及新生儿血型。结果：l0例孕A型 血胎儿的孕妇血浆中扩增出A基因8例，6例孕B型血胎儿的孕妇血浆中扩增出B基因6例， 23例孕O型血胎儿扩增出O基因而无A或B基因2l例，准确性和敏感性分别为89．7％(35／ 39)和87．5％(14／16)。结论：利用孕妇外周血中游离胎儿DNA检测胎儿ABe血型可行，对诊 断和预防新生儿ABe血型不合}生溶血病的发生具有积极意义。 【关键词】 胎儿DNA；ABe血型；无创性产前诊断 中图分类号：R7l4．24 6 文献标识码：A 文章编号：1004—7379(2o07)12—0917—03 Prenatal diagnosis of fetal ABO blood types using fetal DNA in maternal plasma of 0 blood types．Fu Xianhu，Chen Hanping，Xu Xiaoyan，et a1．Department of Gynecology and Ob— s~trics，Tongii Hospital，Tongji Medical College ofHuazhong Universit3’ofScience and Technolo— gY．彤 n12 430030 【Ahstraet】Objective：To explore the feasibility of prenatal diagnosis of fetal ABe blood types th fetal DNA in matemal plasma of O blood types．M酬lm‘ls：Peripheral blood samples were obtained from 39 pregnant women whose blood types were 0(pregnancy 36一dO”weeks)．DNA from maternal plasma Was extracted by phenol／chloroform from plasma ． rhe existence of fetal DNA Was confirmed by amplified fetal SRY gene．Fetal ABo genot~ge Was detected by SSP-PCR．Routine serological method Was used to test ABe phenotype of nDthels and newborns．R阪d ：Among the 39 specimens．10 A gen- otype were detected in 8 cases．6 B genotypes were detected in 6 c．agt~。and 23 O genotypes were detec— ted in 21 cases． The accu!aey and sensitivity were 89．7％(35／39)and 87．5％(14／16)．Conclusion：It iS feasible to detect fetal ABe blood types with fetal DNA in maternal plasma ．and it iS of significance to diagnose and prevent hemol~ic di~ase of new~o／ris． 【Key words】 Fetal DNA；ABe blood types；Noninvasive prenatal diagnosis 母胎ABe血型不合是引起新生儿溶血病的