TLR信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用.pdfVIP

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TLR信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用.pdf

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TLR信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用

目录中文摘要··············································································1 英文摘要··············································································4 研究论文 TLR 信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用前言·············································································7 材料与方法····································································8 结果·············································································18 附图·············································································22 讨论·············································································29 结论·············································································30 参考文献·······································································31 综述 Notch 对固有免疫和适应性免疫的调节 ······························32 致谢···················································································41 个人简历·············································································42 万方数据中文摘要 TLR 信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用摘要目的：呼吸道合胞病毒(RSV)是婴幼儿下呼吸道感染的最重要病原体，接种疫苗是重要的预防措施，福尔马林灭活疫苗(RSV) 因其疫苗增强性免疫病理(VEP)成为呼吸道合胞病毒(RSV)疫苗成功研制的主要障碍。已知TLR 激动剂作为佐剂可以调节疫苗诱导的免疫应答类型。本研究选用三种TLR 激动剂（分别为TLR-9 配体CpG2216，TLR-2 配体 PamCys3 和 TLR-3 配体 poly （I:C))作为佐剂，同时采用 MyD88-/- 小鼠，观察TLR 信号增强或抑制后受到RSV 攻击的小鼠肺组织免疫病理的变化，进而研究TLR 信号在呼吸道合胞病毒疫苗增强性免疫病理中的作用。方法： 1 培养细胞来源RSV 病毒，并测其病毒滴度。 2 制备福尔马林灭活疫苗FI-RSV 及对照组疫苗FI-C 。 3 分别用 FI-C,FI-RSV,FI-RSV+CpG2216,FI-RSV+ PamCys3,FI-RSV+ poly(I:C)免疫C57BL/6 野生型小鼠，用FI-RSV 免疫MyD88-/-C57BL/6 小鼠，共免疫三次，正常对照组不免疫。 4 用ELISA 方法测末次免疫后血清抗体IgG ，观察不同组对体液免疫