血管活性肠肽对大鼠脑缺血损伤的神经保护作用.pdfVIP

2007；27(1 2) 南方医科大学学报(J South Med Univ) ·l801． 血管活性肠肽对大鼠脑缺血损伤的神经保护作用 杨 杰 ，赵朝华 ，钱亦华 ，胡晓丹 ，李 明 ，宗长宏 ( 西安交通大学医学院人体解剖与组织胚胎系，陕西 西 安 710061 2西安医学院人体解剖教研室，陕西 西安 710068) 摘要：目的探讨血管活性肠肽(vIP)对脑缺血损伤的神经保护作用和机制。方法SD大鼠侧脑室内注射vIP，通过管腔 内线栓技术闭塞右侧大脑中动脉诱导建立暂时性局部脑缺血模型。采用TTC染色测定大鼠脑梗死体积：免疫组织化学 染色观察SlOO13表达；双抗夹心ELISA检测血清S10013的含量。结果 VIP注射后大鼠脑梗死体积较对照组明显缩小， 减少约32’3％(P0．05)；S10013阳性细胞体积缩小，数目减少(P0．05)；血清S10013含量也明显降低(P0．05)。结论 VIP 脑内注射可明显减小脑缺血后梗死体积，具有神经保护作用。VIP可以抑制SlOO13的过度表达，这可能是V1P的另一 神经保护机制。 关键词：血管活性肠肽；脑缺血；梗死体积；S10013 中图分类号：R743：R332．8 文献标识码：A 文章编号：l673—4254(2007)l2一l80l一04 Neuroprotective effects of vasoactive intestinal peptide against rat cerebral ischemia YANG Jie’，ZHAO Zhao-hua ，QIAN Yi-hua’，HU Xiao—dan’，LI Ming’，ZONG Chang-hong’ Department of Human Anatomy and Histology and Emb~ology,Xi’an Jiaotong University College of Medicine，Xi’an 7 1 006 1，China； 2Department ofHum an An atomy，Xi’an Medical College，Xi’an 710068，China Abstract：0bjectlve To explore the neuroprotective effect of vasoactive intestinal peptide(vIP)in rat ischemic brain injury， Methods vIP was administered via intracerebroventricular injection in SD rats prior to focal cerebralischemia by intraluminal occlusion ofme middle cerebral artery．The infarct volum e was assessed wim TTC staining．and imrnunohistochemistry was performed to analyze the SlOOl3 expression in the cerebral tissue，with the serum concentrations of SlOOl3 detected by double．antibody sandwich enzyme—linked immunosorbent assay．Results After V1P injection．me relative infarct volume in me rats with cerebral ischemia was significantly reduced by 32．3％as comp~ed with the volum e in the control group on day l fP 0．05)，and the number of S 1 0013一positive cells was significantly decreased in the cerebral tissue(尸0．05)．The ection also resulted in significantly decreased serulTl SlOOl3 concentrations in me rats(P