桂枝茯苓丸诱导肿瘤细胞凋亡的分子机制研究.pdfVIP

王 亡的分子机制研究 第9 桂枝茯苓丸诱导肿瘤细胞凋亡的分子机制研究 王 琪 王亚贤② 官 杰 罗晓庆 (齐齐哈尔医学院免疫教研室，齐齐哈尔161042) 中国图书分类号 R73-361 文献标识码 A 文章编号 1000-484X(2007)09-0811-04 [摘 要] 目的：探讨桂枝茯苓丸诱导肿瘤细胞凋亡的机制，为桂枝茯苓丸(GFW)开发应用提供实验依据。方法：计算 抑瘤率，流式细胞仪测定细胞凋亡率，免疫组化法测定p21 蛋白表达，原位杂交法检测Survivin mRNA表达，电镜观察肿瘤 细胞超微结构。结果：GFW抑瘤率为38．93％，流式细胞仪检测GFW组凋亡率17．79％，与模型组比较，差异有统计学意义 (P0．05)。GFW上调肿瘤细胞p21waf／eip蛋白表达，下调Survivin mRNA表达。GFW组镜下可见瘤细胞以凋亡变化为主。内 膜结构完好，核膜清晰，细胞核固缩，染色质团块状散布核内或边集核膜下，并可见凋亡小体。结论：GFW诱导肿瘤细胞凋亡， 其机制可能与上调p21及下调Survivin mRNA表达密切相关。 [关键词] 桂枝茯苓丸；凋亡；p21 ；Survivin The molecular mechanisms for mouse’s neoplasm apoptosis triggered by lotus GFW WANG Qi，WANG Ya·Xian，GUAN Jie，LUO Xiao·Qing．Department ofImmunology，Qiqihaer Medical College，Qiqi· haer 161042，China [Abstract] Objective：To study on GFW—inducing tumor apoptosis and to find the experimenting basis for utilizing GFw in can· cei theiapy．Methods：S180 mouse sarooma model was used to detect inhibiting effects of GFW on tumor grouth in vim，Morphological changes were observed by electron microscopy and flow cytometer was used for determination apoptosis．The protein expression of p21 and the mRNA expression of Survivin were evaluated by immunohistochemistry and in situ hybridization．Results：For$1 80 sarcoma， the inhibiting rate of GFW was 38．93％ ．Apoptosis Was 17．79％ detected by flow cytomety．Typical apoptotic cells and apoptotic bodies were observed through electron microscope．Chromatin was found gathering as aggregates in nucleus or under nucleus membrane and the nuclear body formed at the same time．Survivin mRNA decreased markedly in the GFW group when compared with the model group(P 0．01)，while the postitive expression of p21 Was obviously higher in GWF group than in the model group(P0．01)．Conclu- sion：GFW induces the tumor cell apoptosis，and the mechanism may be of adjusting expression of p21 and Survivin gene． [Key words] GFW；Apoptosis；p21 ；Survivin 中医认为血瘀是肿瘤的主要成因之一，桂枝茯 镜(德