正常妊娠及子痫前期患者外周血中白介素-12、白介素-18的检测意义.pdfVIP

北京医学2008年第3O卷第1期 · 29 · ． 论著 ． 正常妊娠及子痫前期患者外周血中 白介素一12、白介素一18的检测意义 于松 臧春选 【摘要】 目的 检测非孕妇女、正常妊娠孕妇及子痫前期患者外周血中自介素一12(IL一12)和白介素一18(IL一18) 的水平．探讨Thl／Th2细胞因子在妊娠高血压疾病发病中的作用。方法 孕20周后的子痫前期组34例(轻度子痫前 期 15例，重度子痫前期 19例1，正常妊娠组32例，非孕对照组 16例。取外周血进行流式细胞仪检测，选取CD28表达 量最高者的淋巴细胞作为试验对象。采用ELISA法测定IL一12、I卜18，流式细胞仪测定Th1／Th2细胞比。结果 正常 妊娠组、非孕组及子痫前期组IL一12比较均有显著性差异 0．001)。轻度子痫前期组、重度子痫前期组与正常妊娠 组IL一18比较均无显著性差异 0．051。正常妊娠组与子痫前期组IL一18／／L一12比较有显著性差异 0．001)。IL一 18／／L一12浓度比与Thl／Th2细胞比的相关性比较，呈显著负相关 0．01)。结论 正常妊娠的IL一18／IL一12浓度比 显著高于子痫前期患者．与，Ih1厂rh2细胞比呈反向变化 【关键词】 白介素一18 白介素一12 妊娠 子痫 The ratio of interleukin(IL)-12 and IL-18 secreted by peripheral blood mononuclear cells in normal pregnant and pre-eclamptic patients YU Song．ZANG Chun—yi (Department of Obstetrics，Beijing Obstetrics and Gynecology Hospital，Beijing 100026) 【Abstract】 Objective To investigate the ratio of interleukin (IL)-18to IL一12 secreted by peripheral blood mononuclear cells in normal pregnant and pre—eclamptic patients．Methods IL一18 and IL一12 secreted by non—stimulated peripheral blood mononuclear cells(PBMC)were measured in 32 healthy pregnant women，15 mild pre—eclamptic patients and 19 severe pre-eclamptic patients．Sixteen non-pregnant women were controls．IL-18 and IL-12 level were examined by ELISA．TblJTh2 ratios in PBMC were determined by flow eytometry．Results The ratios of IL-18 to IL-12 were sig— nificantly higher in healthy pregnant women than non—pregnant women．These ratios were significantly lower in severe pre- eclamptic cases than in normal pregnancy subjects，while these ratios in mild