用分离胶真空采血管保存血清对ALT测定结果的影响.pdfVIP

238 Labeled ImmunoassaysClin Med，Dec．2007，Vo1．14，No．4 用分离胶真空采血管保存血清 对ALT测定结果的影响 徐艳存 (湘潭市第一人民医院检验科，湖南湘潭 4l1101) 摘要：观察用分离胶真空采血管保存血清对谷丙转氨酶(ALT)测定结果的影响。采集静脉血，分别置于两管分 离胶真空采血管中，分离血清，立即测定ALT后，用原管保存血清，一管放室温，一管放4℃冰箱保存，连续4天 测定两管血清ALT，对两组下降率进行比较。表明第2、3、4、5天测定结果表明，分离真空胶采血管室温保存血 清ALT明显下降，下降率平均为16．2 、32．2 、47．0 、57．6 ；将第1天和第2、3、4、5天的结果进行配对t检 验，结果有显著性差异(PO．05)；同时测定 4℃冰箱保存血清 AST，平均每天下降率为6．9 、7．7 、1O．2 、 12．2 ，下降率无显著性差异。这表明这种分离胶真空采血管室温保存血清，不能用于第 2天以后检查及复查 ALT。 关键词：分离胶真空采血管； ALT测定； 保存条件 中图分类号：R446．11 2 文献标识码：A 文章编号：1006—1703(2007)04—0238—02 Influence of Serum Preservation with Gel Vacuum Tube on the Result of ALT Assay XU Yan—CUll (The Clinical laboratory of the First Peoples Hospital of Xiangtan．Xiangtan，411101 China) Abstract：To observe the influence of serum preservation with gel vacuum tube on the result of AI T assay，venous blood of each patient was obtained and put into two gel vacuum tubes to get the serum first．Serum ALT was assayed immediately as the value of the l day(original val— ue)．Then one of the tubes was kept in room temperature and another at 4℃ separtely．Serum AI T assay was done from 2“ to the 5 day daily to compare with the l day value．It was shown that the serum AI T level in serum kept in room temperature was decreased daily continuously with the average descending rates of 16．2 ，32．2 ，47．0 and 57．6 of the original value with significant statistical difference(P 0．05)with the original ones，while the average de— scending rates of serum AI T in serum kept in 4 C were obviously lower with the value of 6．9 ，7．7％，10．2 and 12．2 ．It has been concluded that serum AI T assay with serum sepa—