黄瓜白粉病菌对苯醚菌酯敏感基线的建立及室内抗药性风险评价.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 37(6)：660-665(2007) 黄瓜白粉病菌对苯醚茵酯敏感基线的建立及 室内抗药性风险评价 王 敏，韩 平，卢晓红，刘西莉 (中国农业大学农学与生物技术学院，北京100094) 摘要：采用叶碟保湿培养法测定了59株黄瓜白粉病菌对苯醚菌酯的敏感性，并建立了敏感基线。结果表明：苯醚菌酯对 59个菌株的平均EC 为(0．010 5-+0．002 8)~g／mL，其敏感性频率分布呈连续性单峰曲线，没有出现敏感性下降的抗药性 亚群体。因此，该敏感基线可以作为监测田间病原菌对苯醚菌酯敏感性变化的参考。室内采用药剂驯化得到4株抗药性 突变体，其抗性指数均高于150，且抗药性状能够稳定遗传。室内抗药性风险评价显示，4株抗药性突变体在致病力、竞争 力上与亲本菌株相比无明显差异或优于亲本菌株。综合分析表明，黄瓜白粉病菌对苯醚菌酯具有较高的室内抗药性风险。 因此，在该药的使用过程中应当采取相应的抗性治理措施。 关键词：黄瓜白粉病菌；苯醚菌酯；敏感基线；抗药性风险 Determination of sensitivity baseliRe of Sphaerotheca fu／igine to ZJ0712 and as． sessment of Its laboratory resistant risk WANG Min，HAN Ping，LU Xiao．hong，LIU Xi．1i (College of Agriculture and Biotechnology，China Agricultural University，Beijing 100094，China) Abstract：The sensitivity of the 59 Sphaerotheca fufigine strains to ZJ0712 was determined by the leaf discs assay．The results showed that the mean of EC5o values was(0．010 5±0．002 8)~g／mL．The sensitivity fre— quency of fuZigine strains to ZJ0712 distributed as a unimodel cuI弋，e．which showed there was no resistant subpopulation among these strains．Thus．it could be used as baseline—sensitivity of S．fufigine to ZJ0712 for field resistance monitoring．In laboratory，four ZJ0712一resistant mutants were obtained through fungicide adap— tation．The resistance of all mutants was stable and the resistance factor was more than 150．／n vitro，as com— pared to their parental strains，the pathogenicity and the competition of resistant mutants were no significantly difference or excelled them．The results suggested that ZJ0712 was a high risk fungicide in laboratory and ap— propriate precautions against resistance development should be taken． Key words：Sphaerotheca fufigine；ZJ0712；sensitivity baseline；resistance risk 中图分类号：$436．421．12；$482．2 文献标识码：A 文章编号：0412-0914(2007)06-0660-06 苯醚菌酯(开发代号ZJ0712)是浙江省化工研 粉病具有非常卓越的防效，并且能明显促进