鸡胸腺素4 串联体的原核表达、纯化与活性 - journal of northeastern .pdf

第32 卷第10期 东 北 大 学 学 报 ( 自 然 科 学 版 ) Vol. 32, No. 10 2 0 1 1 年 10 月 Journal of Northeastern U ni ersity( Natural Science) O ct. 2 0 1 1 4 1 1 2 王国卿, 郭弄潮 , 刘 丽 ( 1. , 110819; 2. , 150100) : T ( 2T) , Ni- NTA 2T , MTT 4 4 4 . , PET - 32a( + ) - 2T BL21( DE3) , 005 mmol/ L 4 IPT G , 67 mg 2T . 2T T , 4 4 4 2T , T . 4 4 : ; ; ; ; ; 4 : Q 819 : A : 1005-3026( 2011) 10- 15 17-04 Prokaryotic Expression, Purification and Bioactivity of Chicken Thymosin Concatemer 4 WA N G Guo-qing, G UO Nong-chao, L I U Li ( 1. School of Sciences, Northeastern Uni ersity, Sheny ang 110819, China; 2. Heilongjiang T echnology Professional College, Shuangcheng 150100, China. Corresponding author: WA NG Guo-qing , E-mail: w g@ yahoo. com. cn) Abstract: The T concatemer ( 2T ) was synthesized by constructing 2T prokaryotic 4 4 4 expression system. 2T was purified and recombined using Ni-NTA spin column, and its 4 immunocompetence was measured by MTT . U nder the induction of 0. 05 mmol/ L IPT G, 6. 7 mg 2T per gram bacteria w as obtained by expressing PET- 32a( + ) - 2T in competent cell BL2 1 4 4 ( DE3) . No signific