利用ssr 荧光标记构建20 个高粱品种指纹图谱 - 作物学报.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2015, 41(4): 658665 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.00658 利用SSR 荧光标记构建20 个高粱品种指纹图谱 王 瑞1 张福耀1,* 程庆军1 田承华1 凌 亮2 1 / , 030600; 2 , 030031 : , DNA SSR 36 SSR , 20 SSR 36 SSR 235 , 7 , 2~12 20 36 SSR 0.3490~0.8813 0.3144~0.8696, 0.6976 0.657136 SSR 2 , 2 SSR 20SSR , , : ; ; SSR ; Establishment of 20 Sorghum Hybrids Fingerprints Using SSR Fluorescent Marker 1 1,* 1 1 2 WANG Rui , ZHANG Fu-Yao , CHENG Qing-Jun , TIAN Cheng-Hua , and LING Liang 1 Sorghum Institute, Shanxi Academy of Agricultural Sciences / Key Laboratory of Genetic and Germplasm Innovation in Sorghum for Shanxi Pro- vince, Yuci 030600, China; 2 Institute of Edible Fungi, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China Abstract: To establish a standard technical system for purity and authenticity identification of sorghum seeds, it is necessary to construct a DNA fingerprinting database of major sorghum hybrids. The fluorescent marker detection of simple sequence repeat (SSR) was used to establish SSR fingerprints of 20 sorghum hybrids that are popularized in medium and late-maturing areas in China since heterosis has been utilized. Thirty-six pairs of SSR primers were screened and 235 alleles were amplified with the set of primers. The number of alleles per locus ranged from two to twelve with an average of seven. The range of gene diversity and polymorphism information content (PIC) at 36 SSR loci of 20 hybrids were 0.3490–0.8813 and 0.3144–0.8696, with an average of 0.6976 and 0.