高血压肝阳上亢证大鼠模型建立和蛋白质表达分析.pdfVIP

2008年第26卷第3期 四 川 中 医 Vo1．26，No．3，2008 Journal of Sichuan of Traditional Chinese Medieine · 9 · ● 实验研究 ● 高 血 压 肝 阳 上 亢 证 大 鼠 模 型 建 立 和 蛋 白 质 表 达 分 析 张 娴 曾 星△ 段小军 欧润妹 广东省中医院 (广东 广州510120) 摘要：目的：复制高血压肝阳上亢证大鼠模型，探讨高血压肝阳上亢证候相关蛋白表达情况。方法：采用自发性 高血压大鼠 (SHR)，灌服附子汤6周，观察造模前后症状、血压、饮水量，同时应用双向电泳技术分析大鼠的肝脏 蛋白质组变化。结果：自发性高血压大鼠造模6周后，易激惹程度增高、饮水量增加等肝阳上亢证候的表现；SHR组 和肝阳上亢组的蛋白斑点总体分布相似，SHR组共检测到蛋白质点数为716±58个；肝阳上亢组875±19个，仅在肝 阳上亢组表达的蛋白质有27个；有28个点在表达量上有明显变化 (5倍以上)，其中15个点下调，13个点上调。结 论：用自发性高血压大鼠 (SHR)灌服附子汤可建立稳定的高血压肝阳上亢讧候大鼠模型，两组差异表达蛋白可能与 高血压肝阳上亢证候形成相关。 关键词：高血压 肝阳上亢 动物模型 蛋白质组 双向电泳 中图分类号：R 544．1 文献标识码：A 文章编号：1000—3649(2007)03一O0O9—03 Reproduction of a Rat Model of Liver-Yang Hyperactivity Syndrome witll Essential Hypertension and Analysis of Protein Ex- pression in Liver／Zhang Xian／／Guangdong hospital ofTCM(Gnangzhou Guangdong 510120，China) Abstract Objective：The aim is to establish hypertension induced Liver-Yang hyperactivity syndrome(LYI-IS)rat model，and analyze the expression in Liver、 Methods：spontaneous hypertension rats(SHR)were used and 6 weeks adminiswafion of Fuzi decoction was taken．The syndrome，blood pressure and water intake was observed daring the period，meanwhile the protein of rat liver was ana— lyzed by two-dimensional electrophoresis． Result：After admimstmtion of Fuzi decoction for 6 weeks，liver-Yang hyperactivity syndrome appeared-easy to be immted and increase of water intake，ete．The distribution of protein spots was similar in SHR group and model group，which were 716±58 and 875±19 respectively．Among these，27 protein spots expressed only in SHR group，28 protein spots were different in expression level(5 times above)in which 15 spots were decreased and 13 spots were incre